Petursson S R, Chervenick P A
Department of Medicine, University of Pittsburgh School of Medicine, PA 15261.
J Lab Clin Med. 1988 Jan;111(1):110-7.
The effect of pokeweed mitogen-stimulated spleen cell conditioned medium (PWCM) on the proliferation of megakaryocytes and megakaryocyte progenitor cells (CFU-M) was studied with a short-term liquid culture (STLC) system. Adherent and nonadherent cells were sampled daily for acetylcholinesterase-positive cells and CFU-M. The proliferative capacity of CFU-M was determined by culturing cells from STLC in secondary methylcellulose cultures and counting the number of megakaryocytes per colony. Positive dose-related effects were observed between the number of megakaryocytes and CFU-M in liquid culture and the concentration of PWCM in the culture. In contrast, the proliferative capacity of CFU-M was lower in cultures containing high concentrations of PWCM compared with cultures containing low concentrations of PWCM. Furthermore, mean megakaryocyte diameter was significantly smaller in cultures containing high levels of PWCM compared with cultures with low concentrations. These data suggest that at low levels of conditioned medium, megakaryocytopoiesis is characterized by production of fewer CFU-M with a higher proliferative capacity and fewer large megakaryocytes. In turn, high concentrations of PWCM promote the production of a greater number of CFU-M with reduced proliferative capacity and an increased number of small megakaryocytes.
采用短期液体培养（STLC）系统研究了商陆丝裂原刺激的脾细胞条件培养基（PWCM）对巨核细胞和巨核细胞祖细胞（CFU-M）增殖的影响。每天对贴壁细胞和非贴壁细胞进行取样，检测乙酰胆碱酯酶阳性细胞和CFU-M。通过将STLC中的细胞接种于二次甲基纤维素培养物中并计数每个集落中的巨核细胞数量来确定CFU-M的增殖能力。在液体培养中，巨核细胞和CFU-M的数量与培养物中PWCM的浓度之间观察到正剂量相关效应。相反，与含有低浓度PWCM的培养物相比，含有高浓度PWCM的培养物中CFU-M的增殖能力较低。此外，与低浓度培养物相比，含有高水平PWCM的培养物中巨核细胞的平均直径明显更小。这些数据表明，在条件培养基水平较低时，巨核细胞生成的特征是产生较少的具有较高增殖能力的CFU-M和较少的大型巨核细胞。相反，高浓度的PWCM促进产生大量增殖能力降低的CFU-M和数量增加的小型巨核细胞。
