Harbin University of Commerce, Harbin, China.
Harbin University of Commerce, Harbin, China.
J Pharm Biomed Anal. 2021 Feb 20;195:113847. doi: 10.1016/j.jpba.2020.113847. Epub 2020 Dec 13.
As a traditional Chinese medicine of invigorating the kidney, Cuscuta chinensis (CC) can be applied in improving the deficiency of kidney qi in menopausal women and regulating the level of estrogen. Previously, it was found that the ethanol extract of CC had an estrogen-like effect. In this study, the metabolic profile and metabolic pathways of rats in sham, ovariectomized model and CC groups were analyzed using UPLC-TOFMS-based metabolomics and the pattern recognition technology. The serum endogenouse metabolites could be well differentiated in different group, indicating significant differences of metabolic profiles. CC had an reverse adjustment effect on 14 differential metabolites of ovariectomized rats, including sinapyl alcohol, deoxycholic acid, prostaglandin B, prostaglandin I, dihydrosphingosine, choline, pentadecanoic acid, arachidonic acid, 1-stearoyl-Sn-Glycerol-3-Phosphocholine, palmitoleic acid, palmitic acid, vaccenic acid, oleic acid and stearic acid. Furthermore, these differential metabolites were categorized into several major pathways, such as biosynthesis of unsaturated fatty acids, lycerophospholipid metabolism and arachidonic acid metabolism. Therefore, it could be concluded that the estrogen-like effect of CC was related to the lipid metabolism to some extent. The research results provide useful help for the in-depth research and development of CC.
作为一种补肾的中药，菟丝子可用于改善绝经后妇女肾气不足和调节雌激素水平。先前发现，菟丝子的乙醇提取物具有类雌激素作用。在这项研究中，采用 UPLC-TOFMS 代谢组学和模式识别技术分析了假手术组、去卵巢模型组和菟丝子组大鼠的代谢谱和代谢途径。不同组别的血清内源性代谢物可明显区分，表明代谢谱存在显著差异。菟丝子对 14 种去卵巢大鼠差异代谢物具有逆转调节作用，包括丁香酰基醇、脱氧胆酸、前列腺素 B、前列腺素 I、二氢神经鞘氨醇、胆碱、十五烷酸、花生四烯酸、1-硬脂酰基-SN-甘油-3-磷酸胆碱、棕榈油酸、棕榈酸、棉酚酸、油酸和硬脂酸。此外，这些差异代谢物可归类为几个主要途径，如不饱和脂肪酸的生物合成、甘油磷脂代谢和花生四烯酸代谢。因此，可以得出结论，菟丝子的类雌激素作用在某种程度上与脂质代谢有关。研究结果为深入研究和开发菟丝子提供了有益的帮助。
