Liu Sheng-Jin, Ma Yu-Lu, Fang Fang, Wang Rui, Yang Wen-Guo, Shan Chen-Xiao, Bian Yong, Yan Hui, Zhang Zhi-Jie, Duan Jin-Ao
Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization,National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine,State Administration of Traditional Chinese Medicine Key Laboratory of Chinese Medicinal Resources Recycling Utilization,School of Pharmacy,Nanjing University of Chinese Medicine Nanjing 210023,China.
Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences Beijing 100700,China.
Zhongguo Zhong Yao Za Zhi. 2021 Jun;46(12):3133-3143. doi: 10.19540/j.cnki.cjcmm.20210308.202.
To study the effect of mineral Chloriti Lapis on pulmonary metabolites and metabolic pathways in lung tissues of rats with acute exacerbation of chronic obstructive pulmonary disease(AECOPD). The AECOPD rat model of phlegm heat syndrome was replicated by the method of smoking combined with Klebsiella pneumoniae infection. Except for using UPLC-Q-TOF-MS analysis, SPSS 18.0, SIMCA 13.0 and other software were also used for statistical analysis. Through literature search and online database comparison, the differential metabolites were identified, and the possible metabolic pathways were analyzed. After 15 days of administration, PLS-DA analysis was carried out on lung tissue samples of rats in each group. The results showed that the metabolic profiles of lung tissues of rats in each group could be well separated, which indicated that Chloriti Lapis and aminophylline had significant intervention effect on the lung metabolic profile of rats with AECOPD. Moreover, the metabolic profile of Chloriti Lapis group was closer to that of control group, and the intervention effect was better than that of aminophylline group. As a result, 15 potential differential metabolites were identified: phytosphingosine, sphinganine, tetradecanoylcarnitine, L-palmitoylcarnitine, elaidic carnitine, lysoPC[18∶2(9Z,12Z)], lysoPC(16∶0), lysoPC[18∶1(9Z)], lysoPC(18∶0), stearic acid, lysoPC(15∶0), arachidonic acid, docosapentaenoic acid, linoleic acid and palmitic acid. Among them, Chloriti Lapis could significantly improve the levels of 10 differential metabolites of phytosphingosine, tetradecanoylcarnitine, L-palmitoylcarnitine, elaidic carnitine, lysoPC[18∶2(9Z,12Z)], lysoPC(16∶0), lysoPC[18∶1(9Z)], stearic acid, lysoPC(15∶0), and palmitic acid(P<0.05). The intervention effect of Chloriti Lapis group was better than that of aminophylline group. Analysis of metabolic pathways showed that there were 8 possible metabolic pathways that could be affected, and three of the most important metabolic pathways(pathway impact>0.1) were involved: linoleic acid metabolism, arachidonic acid metabolism, and sphingolipid metabolism. Chloriti Lapis had obvious intervention effects on lung tissue-related metabolites and metabolic pathways in rats with AECOPD, and the effect was better than that of aminophyllinne.
研究礞石对慢性阻塞性肺疾病急性加重期（AECOPD）大鼠肺组织代谢产物及代谢途径的影响。采用吸烟联合肺炎克雷伯菌感染的方法复制AECOPD痰热证大鼠模型。除使用超高效液相色谱-四极杆飞行时间质谱联用仪（UPLC-Q-TOF-MS）分析外，还运用SPSS 18.0、SIMCA 13.0等软件进行统计分析。通过文献检索和在线数据库比对，鉴定差异代谢产物，并分析可能的代谢途径。给药15天后，对各组大鼠肺组织样本进行偏最小二乘法判别分析（PLS-DA）。结果显示，各组大鼠肺组织代谢图谱能够很好地分离，表明礞石和氨茶碱对AECOPD大鼠肺代谢图谱有显著干预作用。而且，礞石组的代谢图谱更接近对照组，干预效果优于氨茶碱组。共鉴定出15种潜在差异代谢产物：植物鞘氨醇、二氢鞘氨醇、十四烷酰肉碱、L-棕榈酰肉碱、反油酸肉碱、溶血磷脂酰胆碱[18∶2(9Z,12Z)]、溶血磷脂酰胆碱(16∶0)、溶血磷脂酰胆碱[18∶1(9Z)]、溶血磷脂酰胆碱(18∶0)、硬脂酸、溶血磷脂酰胆碱(15∶0)、花生四烯酸、二十二碳五烯酸、亚油酸和棕榈酸。其中，礞石可显著提高植物鞘氨醇、十四烷酰肉碱、L-棕榈酰肉碱、反油酸肉碱、溶血磷脂酰胆碱[18∶2(9Z,12Z)]、溶血磷脂酰胆碱(16∶0)、溶血磷脂酰胆碱[18∶1(9Z)]、硬脂酸、溶血磷脂酰胆碱(15∶0)和棕榈酸这10种差异代谢产物的水平（P<0.05）。礞石组的干预效果优于氨茶碱组。代谢途径分析表明，可能有8条代谢途径会受到影响，其中最重要的3条代谢途径（通路影响>0.1）涉及：亚油酸代谢、花生四烯酸代谢和鞘脂代谢。礞石对AECOPD大鼠肺组织相关代谢产物及代谢途径有明显干预作用，且效果优于氨茶碱。
