Kirby Institute, UNSW Sydney, NSW, Australia.
Kirby Institute, UNSW Sydney, NSW, Australia; Sexual and Reproductive Health Unit, Papua New Guinea Institute of Medical Research, Goroka, Papua New Guinea.
Pathology. 2021 Feb;53(2):264-266. doi: 10.1016/j.pathol.2020.10.006. Epub 2020 Dec 25.
The Xpert HPV Test is used at point of care for cervical screening in a number of low and middle income countries (LMIC). It is validated for use with ThinPrep-PreservCyt transport medium which has a high methanol content and is therefore classified as a dangerous good for shipping, making cost, transportation and use challenging within LMIC. We compared the performance of ThinPrep against four non-volatile commercially available media for human papillomavirus (HPV) point of care testing. Ten-fold serial dilutions were prepared using three HPV cell lines each positive for 16, 18 or 31 and with each suspended in five different media types. The media types consisted of Phosphate Buffered Saline (ThermoFisher Scientific, USA), Sigma Virocult (Medical Wire and Equipment, UK), MSwab (Copan, Italy) Xpert Transport Media (Cepheid, USA) and ThinPrep-PreservCyt (Hologic, USA). A total of 105 Xpert HPV tests were conducted in a laboratory setting, with seven 10-fold dilutions of each of the three HPV genotypes tested in all five media types. The lowest HPV 10-fold dilution detected for any media, or cell line was the fifth dilution. MSwab was the only medium to detect HPV to the fifth dilution across all three cell types. MSwab transport media may be a suitable alternative to ThinPrep for Xpert HPV point of care testing. A field based, head to head comparison of both media types using the Xpert HPV assay is warranted to confirm these laboratory based findings.
Xpert HPV 检测在一些中低收入国家（LMIC）的护理点用于宫颈筛查。它已通过 ThinPrep-PreservCyt 运输介质验证，该介质甲醇含量高，因此被归类为运输危险货物，这使得在 LMIC 中成本、运输和使用都具有挑战性。我们比较了 ThinPrep 与四种非挥发性市售 HPV 即时检测用介质的性能。使用三种 HPV 细胞系制备了十倍系列稀释液，每种细胞系分别对 16、18 或 31 呈阳性，并且每种细胞系都悬浮在五种不同的介质类型中。介质类型包括磷酸盐缓冲盐水（ThermoFisher Scientific，美国）、Sigma Virocult（Medical Wire and Equipment，英国）、MSwab（Copan，意大利）、Xpert 运输介质（Cepheid，美国）和 ThinPrep-PreservCyt（Hologic，美国）。在实验室环境中进行了总共 105 次 Xpert HPV 检测，对三种 HPV 基因型的每种进行了七种十倍稀释，在所有五种介质类型中进行了测试。对于任何介质或细胞系，检测到的 HPV 最低十倍稀释度为第五稀释度。MSwab 是唯一一种在所有三种细胞类型中都能检测到 HPV 至第五稀释度的介质。MSwab 运输介质可能是 Xpert HPV 即时检测的替代 ThinPrep 介质。需要使用 Xpert HPV 检测进行基于现场的、直接比较这两种介质类型，以确认这些基于实验室的发现。
