Programa de Pós-graduação em Farmacologia, Universidade Federal de Santa Maria (UFSM), RS, Brazil.
Programa de Pós-graduação em Ciência e Tecnologia dos Alimentos, Universidade Federal de Santa Maria (UFSM), RS, Brazil.
Toxicol Lett. 2021 Mar 15;339:23-31. doi: 10.1016/j.toxlet.2020.12.013. Epub 2020 Dec 24.
Interesterified fat (IF) currently substitutes the hydrogenated vegetable fat (HVF) in processed foods. However, the IF consumption impact on the central nervous system (CNS) has been poorly studied. The current study investigated connections between IF chronic consumption and locomotor impairments in early life period and adulthood of rats and access brain molecular targets related to behavior changes in adulthood offspring. During pregnancy and lactation, female rats received soybean oil (SO) or IF and their male pups received the same maternal supplementation from weaning until adulthood. Pups' motor ability and locomotor activity in adulthood were evaluated. In the adult offspring striatum, dopaminergic targets, glial cell line-derived neurotrophic factor (GDFN) and lipid profile were quantified. Pups from IF supplementation group presented impaired learning concerning complex motor skill and sensorimotor behavior. The same animals showed decreased locomotion in adulthood. Moreover, IF group showed decreased immunoreactivity of all dopaminergic targets evaluated and GDNF, along with important changes in FA composition in striatum. This study shows that the brain modifications induce by IF consumption resulted in impaired motor control in pups and decreased locomotion in adult animals. Other studies about health damages induced by IF consumption may have a contribution from our current outcomes.
反式脂肪（IF）目前替代了氢化植物油（HVF）在加工食品中的应用。然而，IF 对中枢神经系统（CNS）的影响尚未得到充分研究。本研究调查了 IF 在大鼠生命早期和成年期的慢性摄入与运动障碍之间的联系，以及与成年后代行为变化相关的大脑分子靶点。在妊娠和哺乳期，雌性大鼠接受大豆油（SO）或 IF，其雄性幼崽从断奶开始接受相同的母体补充，直至成年。评估幼崽的运动能力和成年后的运动活动。在成年后代纹状体中，定量测定多巴胺能靶点、胶质细胞系衍生的神经营养因子（GDNF）和脂质谱。接受 IF 补充的幼崽在学习复杂运动技能和感觉运动行为方面存在障碍。同一批动物在成年后表现出运动减少。此外，IF 组所有评估的多巴胺能靶点和 GDNF 的免疫反应性降低，以及纹状体中 FA 组成发生重要变化。本研究表明，IF 摄入引起的大脑改变导致幼崽运动控制受损，成年动物运动减少。其他关于 IF 摄入引起的健康损害的研究可能与我们目前的结果有关。
