Littoral Environnement et Sociétés (LIENSs), UMR 7266, CNRS-Université de La Rochelle, 2 rue Olympe de Gouges, F-17042 La Rochelle Cedex 01, France.
Littoral Environnement et Sociétés (LIENSs), UMR 7266, CNRS-Université de La Rochelle, 2 rue Olympe de Gouges, F-17042 La Rochelle Cedex 01, France.
Comp Biochem Physiol Part D Genomics Proteomics. 2021 Mar;37:100779. doi: 10.1016/j.cbd.2020.100779. Epub 2020 Dec 17.
The variegated scallop (Mimachlamys varia) is a filter feeder bivalve encountered in marine regions of the Atlantic coast. In particular, it is present in the La Rochelle marina (France), where it is used for the biomonitoring of marine pollution, due to its ability to strongly bioaccumulate pollutants. In this semi-closed environment, contamination generated by port activities leads to an accumulation of both organic and metal pollutants. Zinc is one of these pollutants, present at a dose of up to 150 μg.L. This study investigated the effects of 48 h zinc exposure upon the metabolic profiles of Mimachlamys varia using UHPLC/QToF (ultra-high performance liquid chromatography-quadrupole time-of-flight) tandem mass spectrometry metabolomics. After acclimation in mesocosms recreating in situ conditions, both controls and exposed with Zn (150 μg.L1) bivalves were dissected to recover the gills after 48 h and stored at -80 °C before metabolites extraction. UHPLC/QToF tandem mass spectrometry was performed to study metabolite composition of samples. Statistical analysis of results using multivariate techniques showed a good classification between control and exposed groups. Eleven identified metabolites were found to be down-modulated in exposed scallops. These variations could reflect potential zinc effects on several of the biological processes, such as energy metabolism, osmoregulation and defense against oxidative stress. Among the eleven metabolites highlighted, four were reported for the first time in an aquatic organism exposed to Zn. This study demonstrates once again the diversity of interactions between bivalves and metals and the complexity of the physiological response of marine bivalves to pollutants.
杂色鲍（Mimachlamys varia）是一种在大西洋沿岸海洋地区发现的滤食性双壳贝类。特别是在法国拉罗谢尔的码头（法国），它被用于海洋污染的生物监测，因为它具有强烈的生物积累污染物的能力。在这个半封闭的环境中，港口活动产生的污染导致有机和金属污染物的积累。锌就是其中一种污染物，其浓度高达 150μg/L。本研究使用 UHPLC/QToF（超高效液相色谱-四极杆飞行时间）串联质谱代谢组学研究了 48 小时锌暴露对杂色鲍代谢谱的影响。在模拟原位条件的中试装置中适应后，对照组和暴露于锌（150μg/L）的双壳类动物均被解剖，在 48 小时后回收鳃，并在 -80°C 下储存，以便在提取代谢物之前进行储存。使用 UHPLC/QToF 串联质谱对样品的代谢物组成进行研究。使用多变量技术对结果进行的统计分析表明，对照组和暴露组之间的分类较好。在暴露的扇贝中发现 11 种鉴定出的代谢物下调。这些变化可能反映了锌对几种生物过程的潜在影响，如能量代谢、渗透调节和抗氧化应激防御。在突出的 11 种代谢物中，有 4 种是在水生生物暴露于锌的情况下首次报道的。这项研究再次证明了双壳类动物和金属之间相互作用的多样性，以及海洋双壳类动物对污染物的生理反应的复杂性。
