Department of Obstetrics & Gynecology, Keio University School of Medicine, Tokyo, Japan.
Department of Obstetrics & Gynecology, Keio University School of Medicine, Tokyo, Japan.
Tissue Cell. 2021 Feb;68:101472. doi: 10.1016/j.tice.2020.101472. Epub 2020 Dec 24.
The use of mesenchymal stem cell sheets is a promising strategy for skin regeneration. The injection of dissociated human amniotic fluid stem cells (hAFSCs) was recently found to accelerate cutaneous wound healing with reduced fibrotic scarring, similar to fetal wound healing. However, the use of hAFSCs in applications of cell sheet technology remains limited. The aim of this study was to determine the in vivo efficacy of in vitro-cultured hAFSC sheets in wound healing. The cell sheets were characterized by immunohistochemistry and RT-qPCR and grafted onto full-thickness wounds in BALB/c mice. The wound size was measured, and re-epithelialization, granulation tissue area, and collagen content of the regenerated wound were analyzed histologically. Although the hAFSC sheet contained abundant extracellular matrix molecules and expressed high levels of anti-fibrotic mediators, its grafting did not affect wound closure or the size of the granulation tissue area. In contrast, the organization of type I collagen bundles in the regenerated wound was markedly reduced, while the levels of type III collagen were increased after implantation of the hAFSC sheet. These results suggest that hAFSC sheets can exert anti-fibrotic properties without delaying wound closure.
间充质干细胞片的使用是皮肤再生的一种很有前途的策略。最近发现，分离的人羊膜间充质干细胞（hAFSCs）的注射可以加速皮肤伤口愈合，减少纤维性瘢痕形成，类似于胎儿伤口愈合。然而，hAFSCs 在细胞片技术中的应用仍然有限。本研究旨在确定体外培养的 hAFSC 片在伤口愈合中的体内疗效。通过免疫组织化学和 RT-qPCR 对细胞片进行了表征，并将其移植到 BALB/c 小鼠的全层伤口上。测量了伤口的大小，并对再生伤口的再上皮化、肉芽组织面积和胶原含量进行了组织学分析。尽管 hAFSC 片含有丰富的细胞外基质分子，并表达高水平的抗纤维化介质，但它的移植并不影响伤口闭合或肉芽组织面积的大小。相比之下，在植入 hAFSC 片后，再生伤口中 I 型胶原束的组织明显减少，而 III 型胶原的水平增加。这些结果表明，hAFSC 片可以发挥抗纤维化作用，而不会延迟伤口闭合。
