College of Animal Science and Technology, Inner Mongolia University for Nationalities, Tongliao, Inner Mongolia 028000, China.
College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin 130118, China.
Bioresour Technol. 2021 Mar;323:124517. doi: 10.1016/j.biortech.2020.124517. Epub 2020 Dec 9.
Chlorotetracycline (CTC) is one of the most antibiotics present in cattle manure. In present study, three levels of CTC (0, 20 and 40 mg kg) were added to cattle manure composting systems to investigate its effects on the distribution of antibiotic-resistant genes (ARGs) and succession of bacterial community. Adding CTC hindered the removal of ARGs during composting; the high level of CTC significantly increased the relative abundance (RA) of 9/11 ARGs and four MGEs. The bacterial community could be clustered according to the composting time under various treatments, with the high level of CTC having a more persistent effect on the bacterial community. Based on redundancy analysis, bacterial community explained the most variation in ARGs (50.1%), whereas based on network analysis, Firmicutes and Proteobacteria were the main hosts for ARGs. In conclusion, the presence of CTC increased the risks of spreading ARGs in compost products.
金霉素（CTC）是牛粪中存在的最常见抗生素之一。在本研究中，向牛粪堆肥系统中添加了三个浓度水平的 CTC（0、20 和 40 mg kg），以研究其对抗生素抗性基因（ARGs）分布和细菌群落演替的影响。添加 CTC 会阻碍堆肥过程中 ARGs 的去除；高浓度的 CTC 会显著增加 11 种 ARGs 和 4 种移动遗传元件（MGEs）的相对丰度（RA）。根据不同处理下的堆肥时间，细菌群落可以聚类，高浓度的 CTC 对细菌群落的影响更为持久。基于冗余分析，细菌群落解释了 ARGs 的最大变异（50.1%），而基于网络分析，厚壁菌门和变形菌门是 ARGs 的主要宿主。总之，CTC 的存在增加了 ARGs 在堆肥产品中传播的风险。
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