College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi 712100, China.
College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi 712100, China; Research Center of Recycle Agricultural Engineering and Technology of Shaanxi Province, Northwest A&F University, Yangling, Shaanxi 712100, China.
Bioresour Technol. 2018 Nov;268:20-27. doi: 10.1016/j.biortech.2018.07.086. Epub 2018 Jul 25.
This study investigated the effects of the addition of coal gasification slag (CGS) at three levels (0%, 5%, and 10% w/w) on antibiotic resistance genes (ARGs) and the bacterial community during composting. The addition of CGS effectively facilitated the removal of ARGs and mobile genetic elements (MGEs), where it significantly reduced the relative abundances of 5/11 ARGs and three MGEs in the swine manure composting product. In addition, the enrichment of ARGs and intI1 was lower under the addition of 10% CGS compared with 0% CGS. The bacterial community was distributed according to the composting time under different treatments. Redundancy analysis showed that bacterial community succession and MGE-mediated horizontal gene transfer played important roles in the variations in ARGs. Network analysis indicated the co-occurrence of ARGs and MGEs with specific microorganisms. Thus, 10% CGS may be a suitable additive for reducing the risks of ARGs in compost products.
本研究探讨了在三个水平（0％、5％和 10％w/w）下添加煤气化渣（CGS）对堆肥过程中抗生素抗性基因（ARGs）和细菌群落的影响。添加 CGS 可有效促进 ARGs 和移动遗传元件（MGEs）的去除，显著降低猪粪堆肥产物中 5/11 个 ARGs 和 3 个 MGEs 的相对丰度。此外，与添加 0％CGS 相比，添加 10％CGS 时 ARGs 和 intI1 的富集程度较低。在不同处理下，细菌群落根据堆肥时间分布。冗余分析表明，细菌群落演替和 MGE 介导的水平基因转移在 ARGs 的变化中起重要作用。网络分析表明，ARGs 和 MGEs 与特定微生物的共存。因此，10％CGS 可能是减少堆肥产品中 ARGs 风险的合适添加剂。
