橙皮苷通过上调 miR-146a-5p 对 CORT 诱导的小鼠海马神经细胞凋亡和氧化应激的影响。

Effect of hesperidin on CORT-induced apoptosis and oxidative stress of mouse hippocampal nerve cells by up-regulating miR-146a-5p.

机构信息

Xinhua Community Health Service Center in Wulong Street, Qiqihar, China.

Pathology School, Qiqihar Medical University, Qiqihar, China.

出版信息

Pak J Pharm Sci. 2020 May;33(3(Special)):1383-1388.

Abstract

This study aims to investigate the effect of hesperidin on CORT-induced apoptosis and oxidative stress of mouse hippocampal nerve cells by up-regulating miR-146a-5p and related mechanism. Hesperidin was applied to CORT-induced HT-22 cells, or HT-22 cells whose expression of mir-146a-5p was up-regulated or down-regulated by CORT. The apoptosis rate was detected by flow cytometry. Expression of Cleaved-caspase-3 protein in cells was detected by Western blot. The levels of MDA, SOD and CAT in the cells were detected by enzyme-linked immunosorbent assay, and the expression of miR-146a-5p was detected by RT-qPCR. The application of hesperidin or up-regulation of miR-146a-5p can reduce the CORT-induced apoptosis rate of HT-22 cells, Cleaved caspase-3 protein expression and MAD content (p<0.05), and increase the activity of SOD and CAT and the expression of miR-146a-5p (p<0.05). In contrast, down-regulation ofmiR-146a-5p can increase the CORT-induced apoptosis rate of HT-22 cells, Cleaved caspase-3 protein expression and MAD content (p<0.05), and decrease the activity of SOD and CAT and the expression of miR-146a-5p (p<0.05). Down-regulation of miR-146a-5p expression can reverse the effects of hesperidin on CORT-induced HT-22 cell apoptosis and oxidative stress. Hesperidin may protect cells from being damaged by up-regulating miR-146a-5p to reduce CORT-induced HT-22 cell apoptosis and oxidative stress.

摘要

本研究旨在通过上调 miR-146a-5p 研究橙皮苷对 CORT 诱导的小鼠海马神经细胞凋亡和氧化应激的影响及相关机制。用 CORT 诱导 HT-22 细胞，或用 CORT 上调或下调 mir-146a-5p 表达的 HT-22 细胞应用橙皮苷。通过流式细胞术检测细胞凋亡率。用 Western blot 检测细胞中 Cleaved-caspase-3 蛋白的表达。用酶联免疫吸附试验检测细胞中 MDA、SOD 和 CAT 的水平，用 RT-qPCR 检测 miR-146a-5p 的表达。橙皮苷的应用或 miR-146a-5p 的上调可以降低 CORT 诱导的 HT-22 细胞凋亡率、Cleaved caspase-3 蛋白表达和 MAD 含量（p<0.05），并增加 SOD 和 CAT 的活性和 miR-146a-5p 的表达（p<0.05）。相反，下调 miR-146a-5p 可以增加 CORT 诱导的 HT-22 细胞凋亡率、Cleaved caspase-3 蛋白表达和 MAD 含量（p<0.05），并降低 SOD 和 CAT 的活性和 miR-146a-5p 的表达（p<0.05）。下调 miR-146a-5p 表达可以逆转橙皮苷对 CORT 诱导的 HT-22 细胞凋亡和氧化应激的作用。橙皮苷可能通过上调 miR-146a-5p 来保护细胞免受损伤，从而减轻 CORT 诱导的 HT-22 细胞凋亡和氧化应激。