Gauhati University, Guwahati 781 014, India.
J Genet. 2020;99.
Male fertility in farm animals is considered as an important economic trait. The phenomenon of spermatogenesis plays a dynamic functional role in determining the viability of sperm and thereby can impact on fertility-driven complications. The process of spermatogenesis is controlled by numerous molecular factors and requires a precisely regulated pattern of gene expression. The role of small noncoding RNAs in altering gene expression has been extensively studied. However, limited information is available apropos their role in yak spermatogenesis. The present study aimed to evaluate the assessment of some significant microRNAs and their expression pattern in the body tissues and sperm of fertile and subfertile yak from Arunachal Pradesh besides identified a novel class of sperm enriched small RNA 'mature-sperm-enriched small RNA' (mse-tsRNA) in Yak spermatozoa. The RNAwas extracted from tissue and sperm using 27 gauge needles and subsequently reverse transcribed into small RNA cDNAs. The PCR positive sperm-predominant miRNAs were validated by quantitative reverse transcriptase PCR (qRT-PCR) for their expression in fertile and subfertile yak. Of the 22 microRNAs, the miRNA19a, miRNA142 and miRNA143 showed higher expression in the subfertile yak, whereas expression of miRNA7d, miRNA23a and miRNA23b were found elevated in the fertile animal. The presence of these small noncoding RNAs in yak sperm and testis indicated the legitimate involvement of their role in yak bull fertility.
在农场动物中，雄性生育力被认为是一个重要的经济性状。精子发生现象在决定精子活力方面起着动态的功能作用，从而可能影响与生育力相关的并发症。精子发生过程受许多分子因素控制，需要精确调节基因表达模式。小非编码 RNA 在改变基因表达方面的作用已经得到了广泛的研究。然而，关于它们在牦牛精子发生中的作用的信息有限。本研究旨在评估在不丹从阿萨姆邦采集的有生育力和生育力低下的牦牛的组织和精子中一些重要的 microRNA 及其表达模式，并鉴定牦牛精子中一类新的富含精子的小 RNA“成熟精子富集小 RNA”(mse-tsRNA)。使用 27 号针头从组织和精子中提取 RNA，然后将其反转录成小 RNA cDNA。通过定量逆转录聚合酶链反应 (qRT-PCR) 验证了 PCR 阳性的精子优势 microRNA 在有生育力和生育力低下的牦牛中的表达。在 22 个 microRNA 中，miRNA19a、miRNA142 和 miRNA143 在生育力低下的牦牛中表达较高，而 miRNA7d、miRNA23a 和 miRNA23b 在有生育力的动物中表达升高。这些小非编码 RNA 存在于牦牛精子和睾丸中，表明它们在牦牛生育力中的作用是合法的。
