Debier Cathy, Pirard Laura, Verhaegen Marie, Rzucidlo Caroline, Tinant Gilles, Dewulf Clément, Larondelle Yvan, Smith Donald R, Rees Jean-François, Crocker Daniel E
Louvain Institute of Biomolecular Science and Technology, UCLouvain, Louvain-la-Neuve, Belgium.
Department of Biology, Sonoma State University, Rohnert Park, CA, United States.
Front Physiol. 2020 Dec 10;11:615784. doi: 10.3389/fphys.2020.615784. eCollection 2020.
Adipose tissue plays key roles in energy homeostasis. Understanding its metabolism and regulation is essential to predict the impact of environmental changes on wildlife health, especially in fasting-adapted species. However, experimental work in wild vertebrates can be challenging. We have developed a novel approach of precision-cut adipose tissue slices from northern elephant seal () as a complementary approach to whole animal models. Blubber biopsies were collected from 14 pups during early and late post-weaning fast (Año Nuevo, CA, United States), precision-cut into 1 mm thick slices and maintained in culture at 37°C for at least 63 h. The slices exhibited an efficient response to ß-adrenergic stimulation, even after 2 days of culture, revealing good tissue function. The response to lipolytic stimulus did not vary between regions of outer and inner blubber, but was higher at early than at late fast for inner blubber slices. At early fast, lipolysis significantly reduced leptin production. At this stage, inner blubber slices were also more efficient at producing leptin than outer blubber slices, especially in the non-lipolytic condition. This model will aid the study of adipose tissue metabolism and its response to environmental stressors in marine mammals.
脂肪组织在能量平衡中起着关键作用。了解其代谢和调节对于预测环境变化对野生动物健康的影响至关重要，尤其是在适应禁食的物种中。然而，在野生脊椎动物身上进行实验工作可能具有挑战性。我们开发了一种从北象海豹（）获取精确切割脂肪组织切片的新方法，作为对全动物模型的一种补充方法。在断奶后早期和晚期禁食期间（美国加利福尼亚州阿诺诺夫）从14只幼崽身上采集脂肪活检样本，精确切成1毫米厚的切片，并在37°C下培养至少63小时。这些切片即使在培养2天后仍对β-肾上腺素能刺激表现出有效反应，显示出良好的组织功能。对脂解刺激的反应在皮下脂肪外层和内层区域之间没有差异，但对于内层皮下脂肪切片，在禁食早期比晚期更高。在禁食早期，脂解显著降低了瘦素的产生。在此阶段，内层皮下脂肪切片在产生瘦素方面也比外层皮下脂肪切片更有效，尤其是在非脂解条件下。该模型将有助于研究海洋哺乳动物脂肪组织的代谢及其对环境应激源的反应。
