类固醇生长促进剂对肉鸡肝脏形态和生化适应性的影响。
Effects of steroid growth promoter on morphological and biochemical adaptations in liver of broiler.
作者信息
Sultana Nasrin, Afrose Marzia, Rafiq Kazi
机构信息
Department of Anatomy and Histology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh.
Department of Pharmacology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh.
出版信息
Vet World. 2020 Nov;13(11):2330-2337. doi: 10.14202/vetworld.2020.2330-2337. Epub 2020 Nov 5.
AIM
The study was conducted to observe the effects of dexamethasone (DEX) on the gross study and histomorphometry of liver and on the alterations of biochemical parameters of broilers.
MATERIALS AND METHODS
Ninety day-old chicks were collected and assigned to one of three groups: The control, Group A, and Group B. The control, Group A, and Group B were fed for 28 days with a homemade ration, a commercial broiler type ration, and a homemade ration with DEX (7 mg/kg feed), respectively. Liver samples were collected from the individual birds after sacrificing on days 7, 14, 21, and 28 of the experiment. Morphometric characteristics (length, weight, color, and texture) of the liver were examined. Histomorphological alterations of the liver were assessed with routine hematoxylin and eosin staining. To measure the biochemical parameters, blood samples were collected on days 7, 14, 21, and 28 of the experiment. Liver function test was performed spectrophotometrically by analyzing serum biochemical markers, that is, alanine aminotransferase (ALT), aspartate aminotransferase, and alkaline phosphatase (ALP). Thin-layer chromatography (TLC) was performed for the detection of hepatic steroids.
RESULTS
Hemorrhagic and congested livers were found in broilers of Group B. There were no significant changes found in weight and length of the livers; only numerical decrease in weight and length was observed in birds of Group B. Liver width was increased in Group B on day 21. Histological observation of livers showed accumulation of lipid droplets, congestion of the sinusoids, and central veins in broilers of Group B. Biochemical analyses showed increased levels of ALT in Group B as compared to Group A on day 14 of the experiment. TLC evaluation revealed a positive result in Group B on 28 days of the experiment.
CONCLUSION
The present study results show that DEX may alter the liver morphology and the concentration of ALT in the circulation of broilers.
目的
本研究旨在观察地塞米松（DEX）对肉鸡肝脏大体形态、组织形态计量学以及生化参数变化的影响。
材料与方法
收集90日龄雏鸡并分为三组：对照组、A组和B组。对照组、A组和B组分别用自制日粮、商用肉鸡日粮以及添加DEX（7毫克/千克饲料）的自制日粮喂养28天。在实验的第7、14、21和28天处死个体鸡后采集肝脏样本。检查肝脏的形态计量学特征（长度、重量、颜色和质地）。用常规苏木精和伊红染色评估肝脏的组织形态学改变。为测量生化参数，在实验的第7、14、21和28天采集血液样本。通过分析血清生化标志物，即丙氨酸转氨酶（ALT）、天冬氨酸转氨酶和碱性磷酸酶（ALP），用分光光度法进行肝功能测试。进行薄层色谱法（TLC）检测肝脏类固醇。
结果
B组肉鸡肝脏出现出血和充血。肝脏的重量和长度未发现显著变化；仅观察到B组鸡的肝脏重量和长度有数值下降。在第21天B组肝脏宽度增加。肝脏组织学观察显示B组肉鸡肝脏有脂滴积聚、肝血窦和中央静脉充血。生化分析表明，在实验第14天，B组ALT水平高于A组。TLC评估显示在实验第28天B组结果呈阳性。
结论
本研究结果表明，DEX可能会改变肉鸡的肝脏形态以及循环中ALT的浓度。
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