College of Public Health, Zhengzhou University, Zhengzhou, Henan 450001, People's Republic of China.
College of Chemistry, Zhengzhou University, Zhengzhou, Henan 450001, People's Republic of China.
Int J Nanomedicine. 2020 Dec 14;15:9975-9985. doi: 10.2147/IJN.S286317. eCollection 2020.
As two important tumor markers, vascular endothelial growth factor (VEGF) and carcinoembryonic antigen (CEA) have a great value for clinical application in the early diagnosis of cancer. Due to the complex composition of biological samples, the results from combined detection of CEA and VEGF are often taken as a comprehensive indicator in order to make an accurate judgment on a disease. However, most of the current methods can only be used to detect the content of one biomarker. Therefore, it is necessary to explore a simple, rapid, low-cost, and highly sensitive method for the simultaneous detection of CEA and VEGF.
Based on specific aptamers and magnetic separation, a time-resolved chemiluminescence enzyme-linked aptamer assay was developed for the simultaneous detections of CEA and VEGF in serum samples.
Under the optimal conditions, the linear range of the calibration curve for VEGF was from 0.5 to 80 ng mL, and the limit of detection was 0.1 ng mL. The linear range of the calibration curve for CEA was 0.5 to 160 ng mL, and the limit of detection was 0.1 ng mL. The established method was applied to detect VEGF and CEA in serum samples. The results were consistent with those of commercial kits.
The method has high sensitivity and can quickly obtain accurate results, which could greatly improve the measurement efficiency, reduce the cost, and also reduce the volume of sample consumed. It can be seen that the method established in this study has important application value and broad application prospect in clinical diagnosis.
血管内皮生长因子（VEGF）和癌胚抗原（CEA）作为两种重要的肿瘤标志物，在癌症的早期诊断中具有重要的临床应用价值。由于生物样本组成复杂，通常将 CEA 和 VEGF 的联合检测结果作为综合指标，以对疾病做出准确判断。然而，目前大多数方法只能用于检测一种生物标志物的含量。因此，有必要探索一种简单、快速、低成本、高灵敏度的方法，用于同时检测 CEA 和 VEGF。
基于特异性适体和磁分离，建立了一种时间分辨化学发光酶联适体分析方法，用于血清样本中 CEA 和 VEGF 的同时检测。
在最佳条件下，VEGF 的校准曲线线性范围为 0.5 至 80ng/mL，检测限为 0.1ng/mL。CEA 的校准曲线线性范围为 0.5 至 160ng/mL，检测限为 0.1ng/mL。该方法用于检测血清样本中的 VEGF 和 CEA，结果与商业试剂盒一致。
该方法具有较高的灵敏度，能够快速获得准确的结果，可大大提高测量效率，降低成本，同时减少样品消耗。可以看出，本研究建立的方法在临床诊断中具有重要的应用价值和广阔的应用前景。
