葛根素在高糖环境下对成骨细胞增殖、分化及Ⅰ型胶原mRNA表达的影响
EFFECT OF PUERARIN ON THE PROLIFERATION AND DIFFERENTIATION OF OSTEOBLASTS AND THE EXPRESSION OF TYPE I COLLAGEN mRNA IN A HIGH-GLUCOSE ENVIRONMENT.
作者信息
Wang X H, Shi X W, Luo X X, Zhang D H
机构信息
Gansu Provincial Hospital of Traditional Chinese Medicine, Department of Endocrinology, Lanzhou, China.
出版信息
Acta Endocrinol (Buchar). 2020 Jul-Sep;16(3):288-294. doi: 10.4183/aeb.2020.288.
OBJECTIVE
To investigate the effect of puerarin (Pue) on the proliferation and differentiation of osteoblasts and the expression of type I collagen(Coll I) mRNA in a high-glucose (HG) environment, and to provide evidence for the clinical treatment of diabetic osteoporosis(DOP).
SUBJECTS AND METHODS
The proliferation of osteoblasts from three groups - the control group, the HS group, and the HG+Pue (10-10 M) group - was cultivated for 72 h and evaluated using the methyl thiazolyltetrazolium (MTT) assay.
RESULTS
The MTT values and the ALP activities in all experimental groups were significantly lower than those in the control group, and the MTT values and the ALP activities in the HG+Pue group were significantly higher than those in the HS group. Coll I mRNA expression in all experimental groups was significantly lower than that in the control group, while that in the HG+Pue group was significantly higher than that in the HG group.
CONCLUSIONS
The proliferation and differentiation of osteoblasts and the expression of Coll I mRNA were inhibited by high glucose, but Pue can increase the proliferation and differentiation as well as the expression of Coll I mRNA in the osteoblasts, indicating that Pue could be therapeutically beneficial against DOP.
目的
探讨葛根素（Pue）对高糖（HG）环境下成骨细胞增殖、分化及Ⅰ型胶原蛋白（Coll I）mRNA表达的影响，为糖尿病性骨质疏松症（DOP）的临床治疗提供依据。
对象与方法
将三组成骨细胞——对照组、高糖组（HS组）和高糖+葛根素（10⁻¹⁰ M）组——培养72 h，采用甲基噻唑基四氮唑（MTT）法评估细胞增殖情况。
结果
各实验组的MTT值和碱性磷酸酶（ALP）活性均显著低于对照组，高糖+葛根素组的MTT值和ALP活性显著高于高糖组。各实验组的Coll I mRNA表达均显著低于对照组，而高糖+葛根素组的表达显著高于高糖组。
结论
高糖抑制成骨细胞的增殖、分化及Coll I mRNA的表达，但葛根素可增加成骨细胞的增殖、分化及Coll I mRNA的表达，表明葛根素对糖尿病性骨质疏松症可能具有治疗作用。
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