Ahier Arnaud, Suman Shashi Kumar, Jarriault Sophie
IGBMC, Development and Stem Cells Department, CNRS UMR7104, INSERM U1258, Université de Strasbourg, Illkirch CU Strasbourg, 67404 France.
current address: Clem Jones Centre for Ageing Dementia Research, Queensland Brain Institute, The University of Queensland, Brisbane, Australia.
MicroPubl Biol. 2020 Dec 21;2020. doi: 10.17912/micropub.biology.000339.
Strong loss-of-function or null mutants can sometimes lead to a penetrant early lethality, impairing the study of these genes' function. This is the case for the null mutant, which exhibits 100% penetrant lethality. Here, we describe how we used gene bashing to identify distinct regulatory regions in the locus. This allowed us to generate a null strain that is viable and still displays mutant Y-to-PDA transdifferentiation phenotype. Such strategy can be applied to many other mutants impacting viability.
功能严重丧失或无效突变体有时会导致显性早期致死性，从而妨碍对这些基因功能的研究。无效突变体就是这种情况，它表现出100%的显性致死性。在这里，我们描述了我们如何使用基因敲除来鉴定该基因座中的不同调控区域。这使我们能够产生一种可行的无效菌株，该菌株仍然表现出突变体从Y细胞向胰腺导管腺泡细胞转分化的表型。这种策略可以应用于许多其他影响生存能力的突变体。
