Zhang Ningfeng, Deng Zhiqin, Li Wencui, Zou Yan, Xiong Jianyi, Duan Li, Wang Daping
Key Laboratory of Tissue Engineering, Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, Guangdong 518000, P.R. China.
Exp Ther Med. 2021 Jan;21(1):71. doi: 10.3892/etm.2020.9503. Epub 2020 Nov 25.
The purpose of the present study was to investigate the expression profile of leucine-rich repeat-containing protein 8A (LRRC8A) in osteosarcoma and normal cortical bone, as well as its association with sex, age and tumor malignancy. Immunohistochemical staining of osteosarcoma tissue microarrays (TMAs) was performed to determine the protein expression of LRRC8A and compare them among different subgroups. The expression of LRRC8A in the nuclei and cytoplasm of U2OS tumor cells and MC3T3-E1 osteoblast-like cells was determined using reverse transcription-quantitative PCR. Of all samples of the TMA for patients with osteosarcoma that were tested, 94% featured high cytoplasmic expression of LRRC8A, while in all normal bone tissue control groups, the gene was mainly expressed in the nucleus. In MC3T3-E1 osteoblasts, the expression of LRRC8A at the RNA level was mainly in the cytoplasm. The difference in expression of LRRC8A between microarrays and osteoblasts was statistically significant. In U2OS osteosarcoma cells, LRRC8A mRNA was concentrated in the nuclei and cytoplasm. In osteosarcoma, the expression level of LRRC8A was not significantly associated with sex or age. In conclusion, LRRC8A was highly expressed in the cytoplasm of osteosarcoma cells and the degree of expression may be associated with the degree of tumor malignancy.
本研究的目的是调查富含亮氨酸重复序列蛋白8A（LRRC8A）在骨肉瘤和正常皮质骨中的表达谱，以及其与性别、年龄和肿瘤恶性程度的关系。对骨肉瘤组织芯片（TMA）进行免疫组织化学染色，以确定LRRC8A的蛋白表达，并在不同亚组之间进行比较。使用逆转录定量PCR测定LRRC8A在U2OS肿瘤细胞和MC3T3-E1成骨样细胞的细胞核和细胞质中的表达。在所有检测的骨肉瘤患者TMA样本中，94%的样本LRRC8A在细胞质中高表达，而在所有正常骨组织对照组中，该基因主要在细胞核中表达。在MC3T3-E1成骨细胞中，LRRC8A在RNA水平的表达主要在细胞质中。芯片与成骨细胞中LRRC8A表达的差异具有统计学意义。在U2OS骨肉瘤细胞中，LRRC8A mRNA集中在细胞核和细胞质中。在骨肉瘤中，LRRC8A的表达水平与性别或年龄无显著相关性。总之，LRRC8A在骨肉瘤细胞的细胞质中高表达，表达程度可能与肿瘤恶性程度相关。
