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极度濒危的小安的列斯鬣蜥(爬行纲:鬣蜥科)的完整线粒体基因组。

The complete mitochondrial genome of the critically endangered Lesser Antillean iguana (; Squamata: Iguanidae).

作者信息

Miller Aryeh H, C Jackson Anna, van den Burg Matthijs P, Knapp Charles R, Welch Mark E, Reynolds R Graham

机构信息

Department of Biology, University of North Carolina Asheville, Asheville, NC, USA.

Department of Vertebrate Zoology, Smithsonian Institution, National Museum of Natural History, Washington, DC, USA.

出版信息

Mitochondrial DNA B Resour. 2019 Jul 12;4(2):2479-2481. doi: 10.1080/23802359.2019.1637789.

DOI:10.1080/23802359.2019.1637789
PMID:33365591
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7687459/
Abstract

The Lesser Antillean iguana, Laurenti 1768, is one of the most endangered vertebrate taxa in the West Indies. This species faces significant threats, including introgressive hybridization with the introduced congener . We deploy a combination of off-target sequence capture obtained from Illumina reads and targeted Sanger reads to assemble the mitochondrial genome of . The mitogenome is 16,616 bp in length and is comprised of 13 protein-coding genes, two ribosomal subunits (rRNAs), 22 transfer RNAs, and a control region. Gene order is identical to that of congener and other closely related taxa, absent of any tandem repeat regions. We show the phylogenetic utility of the mitogenome with a maximum-likelihood analysis, which yields a topology concordant with previous studies of iguanine taxa. We are hopeful that this genomic resource will be useful in further informing applied conservation and management for this critically endangered species.

摘要

小安的列斯鬣蜥(Laurenti,1768年)是西印度群岛最濒危的脊椎动物类群之一。该物种面临着重大威胁,包括与引入的同属物种进行渐渗杂交。我们利用从Illumina读数获得的脱靶序列捕获和靶向桑格读数相结合的方法来组装[物种名称未给出]的线粒体基因组。该线粒体基因组长度为16,616 bp,由13个蛋白质编码基因、两个核糖体亚基(rRNA)、22个转运RNA和一个控制区组成。基因顺序与同属物种及其他密切相关类群相同,没有任何串联重复区域。我们通过最大似然分析展示了线粒体基因组的系统发育实用性,其产生的拓扑结构与之前对鬣蜥类群的研究一致。我们希望这一基因组资源将有助于为这种极度濒危物种的应用保护和管理提供更多信息。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1b4b/7687459/99a59d6eccac/TMDN_A_1637789_F0001_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1b4b/7687459/99a59d6eccac/TMDN_A_1637789_F0001_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1b4b/7687459/99a59d6eccac/TMDN_A_1637789_F0001_B.jpg

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本文引用的文献

1
The Lesser Antillean Iguana (Iguana delicatissima) on St. Eustatius: Genetically Depauperate and Threatened by Ongoing Hybridization.圣尤斯特歇斯岛小安的列斯海鬣蜥(Iguana delicatissima):遗传上极度退化,并受到持续杂交的威胁。
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2
PHYLUCE is a software package for the analysis of conserved genomic loci.PHYLUCE 是一个用于分析保守基因组位点的软件包。
Bioinformatics. 2016 Mar 1;32(5):786-8. doi: 10.1093/bioinformatics/btv646. Epub 2015 Nov 2.
3
Genetic Evidence of Hybridization between the Endangered Native Species Iguana delicatissima and the Invasive Iguana iguana (Reptilia, Iguanidae) in the Lesser Antilles: Management Implications.
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PLoS One. 2015 Jun 5;10(6):e0127575. doi: 10.1371/journal.pone.0127575. eCollection 2015.
4
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