Cho Hyunjun, Kim Bo-Mi, Lee Won Young, Rhee Jae-Sung
Division of Polar Life Sciences, Korea Polar Research Institute, Incheon, South Korea.
Unit of Polar Genomics, Korea Polar Research Institute, Incheon, South Korea.
Mitochondrial DNA B Resour. 2019 Sep 2;4(2):2836-2838. doi: 10.1080/23802359.2019.1660594.
The Greenland wolf, as s subspecies of the gray wolf, is native to Greenland. Here, we assembled a complete 16,650 bp genome for the mitochondrion by employing Illumina HiSeq platform. The complete mitochondrial genome contained 13 protein-coding genes (PCGs), 22 transfer RNA (tRNA) genes, two ribosomal RNA (rRNA) genes, and one control region. Overall DNA sequence of the mitochondrion was identical to that of gray wolf , although slight difference was observed in their control regions. The genomic structure of mitochondrion was conserved with the gene arrangements of mitogenomes published in Canidae, and phylogenetic analysis confirmed the sister relationship among sp. This information will provide essential molecular reference to elucidate biogeography, phylogenetic distance, and evolutionary history in gray wolves.
格陵兰狼是灰狼的一个亚种，原产于格陵兰岛。在这里，我们利用Illumina HiSeq平台组装了一个完整的16650bp线粒体基因组。完整的线粒体基因组包含13个蛋白质编码基因（PCGs）、22个转运RNA（tRNA）基因、两个核糖体RNA（rRNA）基因和一个控制区。线粒体的总体DNA序列与灰狼的相同，尽管在它们的控制区观察到了细微差异。线粒体的基因组结构与犬科动物中已发表的有丝分裂基因组的基因排列保守，系统发育分析证实了该物种之间的姐妹关系。这些信息将为阐明灰狼的生物地理学、系统发育距离和进化历史提供重要的分子参考。
