Ricardo Paulo Cseri, Françoso Elaine, Arias Maria Cristina
Departamento de Genética e Biologia Evolutiva - Instituto de Biociências, Universidade de São Paulo, São Paulo, Brazil.
Mitochondrial DNA B Resour. 2019 Dec 12;5(1):108-112. doi: 10.1080/23802359.2019.1697188.
Here we investigated the consequences of PCR amplification errors in the identification of intraindividual mtDNA variation. The bumblebee was chosen as model for the gene amplification tests with two DNA polymerases ( and Q5) presenting different error rates. The amplifications using resulted in a significant increase of singleton haplotypes per individual in comparison to Q5. The sequence characteristics indicated that resulted haplotypes are mostly due to amplification errors. Studies focusing on intraindividual variability should address special attention to the DNA polymerase fidelity to avoid overestimation of heteroplasmic haplotypes.
在此，我们研究了聚合酶链式反应（PCR）扩增错误在个体内线粒体DNA（mtDNA）变异鉴定中的后果。选择大黄蜂作为基因扩增测试的模型，使用两种具有不同错误率的DNA聚合酶（和Q5）。与Q5相比，使用进行的扩增导致每个个体中单个单倍型显著增加。序列特征表明，产生的单倍型主要是由于扩增错误。关注个体内变异性的研究应特别注意DNA聚合酶的保真度，以避免高估异质性单倍型。
