Zhao Yang, Shao Heping, Zhang Ningning, Jing Jinquan
Nanjing Institute of Agricultural Sciences in Jiangsu Hilly Area, Nanjing, P.R. China.
Mitochondrial DNA B Resour. 2020 Jan 29;5(1):879-880. doi: 10.1080/23802359.2020.1717386.
The complete mitochondrial genome of Nakahara, 1915 was sequenced in this study. The complete mitochondrial genome is a typical double-stranded circular molecule of 18,585 bp (GenBank accession number: MN852445), containing 37 typical animal mitochondrial gene and an A + T-rich region. The gene order is identical to that of the putative ancestral arrangement of insects and other lacewings. 13 protein-coding genes (PCGs) possessed common triplet initiation codons ATN except possessed TTG and mostly terminated with TAN codons except for and with a single T residue adjacent to a downstream tRNA gene. All of the 22 tRNAs, ranging from 63 to 72 bp, can be folded into classic clover-leaf secondary structure except for , in which the dihydrouridine (DHU) arm did not form a stable stem-loop structure. The control region is 1416 bp long with an A + T content of 90.3%. In the sampled families of Neuroptera, each family showed a monophyletic cluster and Polystoechotidae + Rapismatidae, Osmylidae + the remaining families, Hemerobiidae + (Chrysopidae + (Polystoechotidae + Rapismatidae)) are recovered in phylogenetic analyses with high supports.
本研究对1915年中原的完整线粒体基因组进行了测序。完整的线粒体基因组是一个典型的双链环状分子，长度为18,585 bp（GenBank登录号：MN852445），包含37个典型的动物线粒体基因和一个富含A+T的区域。基因排列顺序与推测的昆虫和其他草蛉祖先排列相同。13个蛋白质编码基因（PCGs）除 具有TTG外，都具有常见的三联体起始密码子ATN，并且除 和 以单个T残基位于下游tRNA基因相邻处外，大多以TAN密码子终止。22个tRNA长度从63到72 bp不等，除 外都能折叠成经典的三叶草二级结构，其中二氢尿嘧啶（DHU）臂未形成稳定的茎环结构。控制区长度为1416 bp，A+T含量为90.3%。在脉翅目采样的科中，每个科都显示为一个单系类群，并且在系统发育分析中，多栉蛉科+瘤蛉科、栉角蛉科+其余科、褐蛉科+（草蛉科+（多栉蛉科+瘤蛉科））得到了高支持率的恢复。
