A Highly Efficient -Mediated Method for Transient Gene Expression and Functional Studies in Multiple Plant Species.

Although the use of stable transformation technology has led to great insight into gene function, its application in high-throughput studies remains arduous. Agro-infiltration have been widely used in species such as for the rapid detection of gene expression and protein interaction analysis, but this technique does not work efficiently in other plant species, including . As an efficient high-throughput transient expression system is currently lacking in the model plant species , we developed a method that is characterized by high efficiency, reproducibility, and suitability for transient expression of a variety of functional proteins in and 7 other plant species, including , , , , , , and . Efficiency of this method was independently verified in three independent research facilities, pointing to the robustness of this technique. Furthermore, in addition to demonstrating the utility of this technique in a range of species, we also present a case study employing this method to assess protein-protein interactions in the sucrose biosynthesis pathway in .