Mo Guichun, He Xuanming, Qin Dongmiao, Jiang Xiaohua, Zheng Xiangfei, Deng Biyang
State Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin 541004, China.
Analyst. 2021 Feb 22;146(4):1334-1339. doi: 10.1039/d0an02106g.
An electrochemiluminescence resonance energy transfer (ECL-RET) immunosensor was developed based on the potential-resolved technology for the simultaneous detection of neuron-specific enolase (NSE) and the cytokeratin 19 fragment (CYFRA21-1). The absorption spectrum of gold nanorods (AuNRs) perfectly overlapped with the ECL spectra of SnS2@Pt and Ru(bpy)32+/Zn-MOF, so they exhibited an excellent ECL-RET effect with high efficiency. Zn-MOF possesses a large surface area, which allows for the loading of Ru(bpy)32+. This results in a signal probe of Ru(bpy)32+/Zn-MOF/Ab1 showing a strong ECL emission. Simultaneously, owing to the excellent electronic conductivity of PtNPs, they can increase the electron transfer rate between S2O82- and tin disulfide nanoflowers (SnS2NFs). Hence, the ECL signal of SnS2NFs can be enhanced. Under the optimal conditions, the linear range for NSE is 0.2 pg mL-1-20 ng mL-1 with a detection limit of 79 fg mL-1. The linear range for CYFRA21-1 is 1.25 pg mL-1-12.5 ng mL-1 with a detection limit of 0.43 pg mL-1. The proposed immunosensor can be used for the sensitive simultaneous detection of NSE and CYFRA21-1 in human serum and has promise for clinical diagnostics.
基于电位分辨技术开发了一种电化学发光共振能量转移（ECL-RET）免疫传感器，用于同时检测神经元特异性烯醇化酶（NSE）和细胞角蛋白19片段（CYFRA21-1）。金纳米棒（AuNRs）的吸收光谱与SnS2@Pt和Ru(bpy)32+/Zn-MOF的ECL光谱完美重叠，因此它们表现出优异的高效ECL-RET效应。Zn-MOF具有较大的表面积，可用于负载Ru(bpy)32+。这导致Ru(bpy)32+/Zn-MOF/Ab1信号探针表现出强烈的ECL发射。同时，由于PtNPs具有优异的电子导电性，它们可以提高S2O82-与二硫化锡纳米花（SnS2NFs）之间的电子转移速率。因此，SnS2NFs的ECL信号可以增强。在最佳条件下，NSE的线性范围为0.2 pg mL-1至20 ng mL-1，检测限为79 fg mL-1。CYFRA21-1的线性范围为1.25 pg mL-1至12.5 ng mL-1，检测限为0.43 pg mL-1。所提出的免疫传感器可用于灵敏地同时检测人血清中的NSE和CYFRA21-1，具有临床诊断应用前景。
