State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin 541004, China.
Analyst. 2023 Sep 11;148(18):4539-4547. doi: 10.1039/d3an01106b.
The construction of advanced systems capable of accurately detecting neuron-specific enolase (NSE) is essential for rapidly diagnosing small-cell lung cancer. In this study, an electrochemiluminescence (ECL) resonance energy transfer immunosensor was proposed for the ultra-sensitive detection of NSE. The co-reactants CO and Ru(bpy) were integrated to form a self-enhanced ECL luminophore (Ru-ZnMOF) as the ECL donor. The abundant carboxyl functional groups of Ru-ZnMOF supported antibody 1 an amidation reaction. Polydopamine-modified zinc dioxide nanoflowers, as ECL acceptors, inhibited Ru-ZnMOF ECL signaling. The linear range of NSE was 10 fg mL to 100 ng mL with a detection limit of 3.3 fg mL (S/N = 3), which is suitably low for determining NSE in real samples.
构建能够准确检测神经元特异性烯醇化酶 (NSE) 的先进系统对于快速诊断小细胞肺癌至关重要。在这项研究中，提出了一种用于超灵敏检测 NSE 的电化学发光（ECL）共振能量转移免疫传感器。将共反应物 CO 和 Ru(bpy) 整合形成自增强 ECL 发光体（Ru-ZnMOF）作为 ECL 供体。Ru-ZnMOF 丰富的羧基官能团支持抗体 1 进行酰胺化反应。聚多巴胺修饰的氧化锌纳米花作为 ECL 受体，抑制了 Ru-ZnMOF 的 ECL 信号。NSE 的线性范围为 10 fg mL 至 100 ng mL，检测限为 3.3 fg mL（S/N = 3），非常适合用于测定实际样品中的 NSE。
