Department of Medical Genetics, School of Basic Medical Sciences, Southern Medical University, Guangzhou, Guangdong, China.
Guangdong Genetics Testing Engineering Research Center, Guangzhou, Guangdong, China.
Br J Haematol. 2021 Apr;193(2):401-405. doi: 10.1111/bjh.17134. Epub 2020 Dec 24.
Krüppel-like factors (KLFs) are a highly conserved family of transcription factors. We analysed expression profile data of KLFs and identified KLF6 as a new potential regulator of erythropoiesis. Knocking down the expression of KLF6 significantly raised γ-globin mRNA and protein levels in the erythroid cell line HUDEP-2 and haematopoietic progenitor (CD34 ) cells. We found that overexpression of microRNA (miR)-2355-5p in HUDEP-2 and CD34 cells correlated with increased γ-globin synthesis by suppressing expression of KLF6. Our discovery that the interaction between miR-2355-5p and KLF6 affects the expression of γ-globin may provide more information for the clinical management of β-thalassaemia patients.
Krüppel 样因子（KLFs）是一类高度保守的转录因子家族。我们分析了 KLFs 的表达谱数据，发现 KLF6 是一种新的红细胞生成潜在调控因子。敲低 KLF6 的表达水平可显著提高红系细胞系 HUDEP-2 和造血祖细胞（CD34）中的 γ-珠蛋白 mRNA 和蛋白水平。我们发现，HUDEP-2 和 CD34 细胞中 microRNA（miR）-2355-5p 的过表达通过抑制 KLF6 的表达与增加 γ-珠蛋白的合成相关。我们的发现，miR-2355-5p 和 KLF6 之间的相互作用影响 γ-珠蛋白的表达，可能为β-地中海贫血患者的临床治疗提供更多信息。
