Eco-Friendly Agri-Bio Research Center, Jeonnam Bioindustry Foundation, Gokseong, Jeonnam, Republic of Korea.
School of Applied Science in National Resources and Environment, Hankyong National University, Anseong, 17579, Korea.
Biomed Chromatogr. 2021 May;35(5):e5057. doi: 10.1002/bmc.5057. Epub 2021 Jan 12.
To promote exports, import tolerance (IT) of thiacloprid in strawberry was proposed using the Organization for Economic Cooperation and Development (OECD) maximum residue limit (MRL) calculator after conducting three different field trials. The pre-harvest interval of residual pattern and degradation dynamics of thiacloprid in strawberry were determined using ultra-performance liquid chromatography-tandem mass spectrometry. Samples were extracted with acetonitrile and a mixture of salts and dilution was performed for purification. A six-point matrix-matched calibration curve was constructed which provided excellent linearity with coefficient of determination (R ) of 0.9998 or more. Detection and quantification limits were 0.003 and 0.01 mg/kg, respectively. The method was validated in quintuplicate at three different concentrations, which resulted in acceptable recovery ranging from 80.86% to 101.71% with relative standard deviation of 6.50 or less among the three field sites. The developed method was applied to the field-treated sample harvested at different intervals. In the pre-harvest interval trial, the amount of thiacloprid residues ranged from 0.24 to 0.70 mg/kg in field site 1 (Nonsan), 0.16 to 0.50 mg/kg in field site 2 (Sunchang), and 0.36 to 0.50 mg/kg in field site 3 (Sacheon). By contrast, in the degradation trial, the observed residues were 0.03-0.81 mg/kg in field site 1 and 0.02-0.48 mg/kg in field site 2. Consequently, the IT of thiacloprid in strawberry using the OECD MRL calculator was proposed as 2 mg/kg, which is exactly the same as the MRL established by the Republic of Korea. In conclusion, the residue study proposes 2.0 mg/kg as the MRL of thiacloprid in strawberries.
为了促进出口，在进行了三次不同的田间试验后，提议使用经济合作与发展组织（OECD）最大残留限量（MRL）计算器来提高噻虫啉在草莓中的进口容忍度（IT）。使用超高效液相色谱-串联质谱法确定噻虫啉在草莓中的残留模式的预收获间隔和降解动态。样品用乙腈和盐混合物提取，并进行稀释以进行净化。构建了一个六点基质匹配校准曲线，其具有优异的线性，决定系数（R）大于或等于 0.9998。检测限和定量限分别为 0.003 和 0.01 mg/kg。该方法在三个不同浓度下进行了五次重复验证，结果表明回收率在 80.86%至 101.71%之间，相对标准偏差（RSD）在三个田间地点均小于 6.50。该方法应用于在不同时间间隔收获的田间处理样品。在预收获间隔试验中，噻虫啉残留量在大田 1（论山）为 0.24 至 0.70 mg/kg，大田 2（顺昌）为 0.16 至 0.50 mg/kg，大田 3（泗川）为 0.36 至 0.50 mg/kg。相比之下，在降解试验中，大田 1 的残留量为 0.03-0.81 mg/kg，大田 2 的残留量为 0.02-0.48 mg/kg。因此，使用 OECD MRL 计算器，噻虫啉在草莓中的 IT 被提议为 2 mg/kg，这与韩国建立的 MRL 完全相同。总之，残留研究建议将噻虫啉在草莓中的 MRL 设定为 2.0 mg/kg。
