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转录因子 FOXA3 通过调节 HNF1A、AFP 和 ZFHX3 的表达促进肝癌的发生。

Transcription factor FOXA3 promotes the development of Hepatoblastoma via regulating HNF1A, AFP, and ZFHX3 expression.

机构信息

Department of Childhood Hematology, Maternal and Child Hospital of Hubei province, Tongji Medical College Huazhong University of Science and Technology, Wuhan, China.

出版信息

J Clin Lab Anal. 2021 Mar;35(3):e23686. doi: 10.1002/jcla.23686. Epub 2020 Dec 24.

DOI:10.1002/jcla.23686
PMID:33368532
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7957975/
Abstract

OBJECTIVE

In this research paper, we aimed to study the role of FOXA3 in hepatoblastoma (HB) and the molecular mechanism.

METHODS

Immunohistochemistry was applied to determine the expression situation of FOXA3 and AFP in HB tissues and the adjacent normal tissues. FOXA3, HNF1A, and ZFHX3 expressions in HB tissues and the normal tissues were measured by Western blot. HB cell lines were randomly divided into 4 groups: Model, si-NC, si-FOXA3-1, and si-FOXA3-2 group. The HB cell viability and colony formation characteristics in the 4 groups were explored by CCK-8 and cell cloning formation assay, respectively. The expression of FOXA3, AFP, HNF1A, ZFHX3, and MYC in HB cells after knockdown of FOXA3 was measured.

RESULTS

FOXA3, AFP, and HNF1A expressions were significantly up-regulated in HB tissues, while ZFHX3 expression was down-regulated. Knockdown of FOXA3 markedly inhibited HB cell viability and cloning formation ability. Knockdown of FOXA3 decreased FOXA3, AFP, and HNF1A/MYC expression, while increased ZFHX3 expression.

CONCLUSION

FOXA3 promotes the occurrence and development of HB by up-regulating AFP and HNF1A/MYC expression, and down-regulating ZFHX3 expression.

摘要

目的

在本研究论文中，我们旨在研究 FOXA3 在肝母细胞瘤（HB）中的作用和分子机制。

方法

应用免疫组织化学法检测 FOXA3 和 AFP 在 HB 组织及相邻正常组织中的表达情况。Western blot 法检测 HB 组织及正常组织中 FOXA3、HNF1A 和 ZFHX3 的表达。将 HB 细胞系随机分为 4 组：模型组、si-NC 组、si-FOXA3-1 组和 si-FOXA3-2 组。分别采用 CCK-8 法和细胞克隆形成实验检测 4 组 HB 细胞的活力和克隆形成特性。检测敲低 FOXA3 后 HB 细胞中 FOXA3、AFP、HNF1A、ZFHX3 和 MYC 的表达。

结果

FOXA3、AFP 和 HNF1A 的表达在 HB 组织中显著上调，而 ZFHX3 的表达下调。敲低 FOXA3 显著抑制 HB 细胞的活力和克隆形成能力。敲低 FOXA3 降低了 FOXA3、AFP 和 HNF1A/MYC 的表达，同时增加了 ZFHX3 的表达。

结论

FOXA3 通过上调 AFP 和 HNF1A/MYC 的表达，下调 ZFHX3 的表达，促进 HB 的发生发展。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/773b/7957975/289bfc924237/JCLA-35-e23686-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/773b/7957975/487b89ec1065/JCLA-35-e23686-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/773b/7957975/36252bf1bb8a/JCLA-35-e23686-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/773b/7957975/608202d3bced/JCLA-35-e23686-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/773b/7957975/6fbc39bd6131/JCLA-35-e23686-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/773b/7957975/e8b731d0490c/JCLA-35-e23686-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/773b/7957975/289bfc924237/JCLA-35-e23686-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/773b/7957975/487b89ec1065/JCLA-35-e23686-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/773b/7957975/36252bf1bb8a/JCLA-35-e23686-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/773b/7957975/608202d3bced/JCLA-35-e23686-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/773b/7957975/6fbc39bd6131/JCLA-35-e23686-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/773b/7957975/e8b731d0490c/JCLA-35-e23686-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/773b/7957975/289bfc924237/JCLA-35-e23686-g003.jpg

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