Archondakis Stavros, Roma Maria, Kaladelfou Evropi
Department of Cytopathology, Alpha Prolipsis Medical Laboratories, Athens, Greece.
Cytopathology. 2021 May;32(3):338-343. doi: 10.1111/cyt.12948. Epub 2020 Dec 24.
The objective of this study was to investigate the feasibility of implementing short videos captured by static telecytological applications for remote evaluation of cervical smears prepared by means of liquid-based cytology.
The study was performed on representative short videos captured from a total of 404 cervical smears (benign, 135; atypical squamous cells of undetermined significance, 92; low-grade squamous intraepithelial lesion, 62; high-grade squamous intraepithelial lesion, 87; squamous cell carcinoma, 26; adenocarcinoma, 2) that were sent via file transfer protocol to password-protected accounts for remote review by three independent cytopathologists. In addition to diagnosis, reviewers commented on the overall digital video quality. Contributors' and reviewers' diagnoses were collected, recorded, and statistically evaluated.
Statistical evaluation detected no significant difference in diagnostic accuracy between cytological diagnoses based on short videos versus conventional slides. The overall interobserver agreement ranged from substantial to almost perfect with κ values of 0.74-0.91.
Short videos produced by static telecytology applications can be used as an alternative method for telecytological diagnosis of cervical smears, particularly for quality control purposes. It is a prompt and valid method for quality assessment and proficiency testing and can be integrated into the daily workflow. Short pre-captured videos of cervical smears can be used for rapid and accurate diagnosis, diminishing turnaround times and improving small cytology departments' quality indices. They can also be used for archiving, teleconsultation, and second opinion purposes, improving the performance of already existing static telecytology stations.
本研究的目的是探讨通过静态远程细胞学应用程序拍摄短视频对液基细胞学制备的宫颈涂片进行远程评估的可行性。
对从总共404例宫颈涂片中拍摄的代表性短视频进行研究（良性，135例；意义不明确的非典型鳞状细胞，92例；低级别鳞状上皮内病变，62例；高级别鳞状上皮内病变，87例；鳞状细胞癌，26例；腺癌，2例），这些涂片通过文件传输协议发送到受密码保护的账户，供三名独立的细胞病理学家进行远程评估。除了诊断外，评估人员还对数字视频的整体质量进行了评价。收集、记录并对贡献者和评估人员的诊断进行了统计评估。
统计评估发现，基于短视频的细胞学诊断与传统玻片诊断在诊断准确性上无显著差异。观察者间的总体一致性从实质性到几乎完美，κ值为0.74 - 0.91。
静态远程细胞学应用程序生成的短视频可作为宫颈涂片远程细胞学诊断的替代方法，特别是用于质量控制目的。它是一种快速有效的质量评估和能力验证方法，可整合到日常工作流程中。预先拍摄的宫颈涂片短视频可用于快速准确的诊断，减少周转时间并改善小型细胞学部门的质量指标。它们还可用于存档、远程会诊和获取第二意见，提高现有静态远程细胞学工作站的性能。
