褪黑素对创伤性脑损伤大鼠皮质神经元再生的影响。
Effect of melatonin on regeneration of cortical neurons in rats with traumatic brain injury.
作者信息
Ge Jianbin, Chen Dandan, Ben Jingjing, Song Xinjian, Zou Linqing, Yi Xin
机构信息
Department of Pharmacy, the Second People's Hospital of Nantong, Nantong, China.
Department of Rehabilitation Medicine, the Second People's Hospital of Nantong, Nantong, China.
出版信息
Clin Invest Med. 2020 Dec 27;43(4):E8-16. doi: 10.25011/cim.v43i4.34829.
PURPOSE
To investigate the effect of melatonin on regeneration of cortical neurons in rats with traumatic brain injury (TBI).
METHODS
Sprague-Dawley rats (n=36) were randomly divided into sham, TBI+vehicle and TBI+melatonin groups. Cerebral blood flow and cognitive function were observed via laser Doppler flowmetry and by Morris water maze testing, respectively. The serum malondialdehyde (MDA) and superoxide dismutase (SOD) levels were used to assess oxidative stress. Immunofluorescence and terminal deoxynucleotidyl transferase dUTP nick end labelling assay was used to observe the newborn neurons and apoptotic cells.
RESULTS
Cerebral blood flow in the TBI+melatonin group was higher than that of the TBI+vehicle group at one, 12, 24 and 48 h post-injury, but the difference was not statistically significant (P>0.05). The cognitive function of the rats was better in the TBI+melatonin group than the TBI+vehicle group (P.
目的
探讨褪黑素对创伤性脑损伤（TBI）大鼠皮质神经元再生的影响。
方法
将36只Sprague-Dawley大鼠随机分为假手术组、TBI+溶剂组和TBI+褪黑素组。分别通过激光多普勒血流仪和Morris水迷宫试验观察脑血流量和认知功能。采用血清丙二醛（MDA）和超氧化物歧化酶（SOD）水平评估氧化应激。通过免疫荧光和末端脱氧核苷酸转移酶dUTP缺口末端标记法观察新生神经元和凋亡细胞。
结果
TBI+褪黑素组在伤后1、12、24和48小时的脑血流量高于TBI+溶剂组，但差异无统计学意义（P>0.05）。TBI+褪黑素组大鼠的认知功能优于TBI+溶剂组（P。 （注：原文此处“P.”表述不完整）
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