Instituto Nacional de Tecnología Agropecuaria (INTA), Instituto Tecnología de Alimentos, Argentina; Instituto de Ciencia y Tecnología de Sistemas Alimentarios Sustentables (UEDD INTA CONICET), Argentina.
Comisión Nacional de Energía Atómica (CNEA), Centro Atómico Ezeiza, Argentina.
Meat Sci. 2021 Apr;174:108414. doi: 10.1016/j.meatsci.2020.108414. Epub 2020 Dec 25.
The aims of the present study were: a) to estimate the minimal dose of gamma irradiation required to reduce 5 log CFU/g of native O157 and non-O157 Shiga toxin-producing Escherichia coli population in ground beef samples inoculated with high inoculum; b) to assess its effectiveness in samples with low inoculum and 3) to evaluate consumer acceptance. Based on the results, 1 kGy was estimated as the minimal dose of gamma irradiation required to reduce 5 log CFU/g of STEC in ground beef. However, when samples with low inoculum level were subjected to 1 kGy, 3.9% of the samples were positive for stx and eae genes after an enrichment step. Consumer acceptance analysis was carried out with samples subjected to 2.5 kGy and no significant differences were found between irradiated and control samples. Therefore, 2.5 kGy was identified as the gama irradiation dose that reduces STEC but has no impact on consumer acceptance of ground beef.
a) 评估减少接种高浓度污染菌的碎牛肉中天然 O157 和非 O157 产志贺毒素大肠杆菌群体 5 个对数 CFU/g 所需的最小辐照剂量；b) 评估其在低浓度接种样品中的效果；c) 评估消费者接受度。基于结果，估计 1 kGy 是减少碎牛肉中 STEC 5 个对数 CFU/g 所需的最小 γ 辐照剂量。然而，当用低浓度接种水平的样品进行 1 kGy 处理时，在富集步骤后，有 3.9%的样品 stx 和 eae 基因呈阳性。用 2.5 kGy 处理的样品进行了消费者接受度分析，辐照和对照样品之间没有发现显著差异。因此，确定 2.5 kGy 为减少 STEC 但对碎牛肉消费者接受度没有影响的 γ 辐照剂量。
