Department of Histology and Embryology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-719 Olsztyn, Poland.
Int J Mol Sci. 2020 Dec 21;21(24):9750. doi: 10.3390/ijms21249750.
The metabolism of pineal indoles is closely related to alterations in the light and dark phases of a daily cycle. Recent research showed important interspecies differences in the pineal biochemistry, and a strong impact of monochromatic light on many physiological processes in birds. Therefore, the aims of study were to characterize the metabolism of melatonin-synthesis indoles in the pineal organ of the domestic turkey, and to determine the changes occurring in this metabolism under the influence of different wavelengths and intensities of light. For this purpose, 3-week-old turkeys were kept under 16 lx white light, or under blue, green, and red light with intensities of 16, 32, and 64 lx during the photophase, and after 7 d were sacrificed at 4 h intervals. The activities of melatonin-synthesizing enzymes and the contents of indoles were measured in the same pineal organ. The results revealed that the activities of tryptophan hydroxylase and arylalkylamine N-acetyltransferase, and the levels of all tryptophan derivatives had significant daily changes in birds kept under each light condition used. The profile of pineal indole metabolism in 4-week-old turkeys was characterized by high-amplitude rhythms in the activity of arylalkylamine N-acetyltransferase and the contents of N-acetylserotonin and melatonin, equal relative amounts of serotonin and 5-hydroxyindoleacetic acid, and higher content of melatonin than N-acetylserotonin. The monochromatic light significantly modified the pineal indole metabolism, and its effects were dependent on the color and intensity of light. Pronounced changes occurred in the level of serotonin synthesis and the daily rhythm course of melatonin synthesis.
松果腺吲哚的代谢与昼夜节律的光暗变化密切相关。最近的研究表明，不同物种的松果腺生物化学存在重要差异，单色光对鸟类的许多生理过程有强烈影响。因此，本研究的目的是表征火鸡松果腺中褪黑素合成吲哚的代谢，并确定在不同波长和强度的光影响下，这种代谢发生的变化。为此，将 3 周龄火鸡在 16 lx 的白光下或在光期内的 16、32 和 64 lx 的蓝光、绿光和红光下饲养 7 天，然后每隔 4 小时处死。在同一松果腺中测量褪黑素合成酶的活性和吲哚的含量。结果表明，在每种光照条件下饲养的鸟类的松果腺中，色氨酸羟化酶和芳基烷基胺 N-乙酰转移酶的活性以及所有色氨酸衍生物的含量均有明显的日变化。4 周龄火鸡的松果腺吲哚代谢特征是芳基烷基胺 N-乙酰转移酶的活性、N-乙酰血清素和褪黑素的含量具有高振幅节律，血清素和 5-羟色胺乙酸的相对含量相等，以及褪黑素的含量高于 N-乙酰血清素。单色光显著改变了松果腺吲哚代谢，其影响取决于光的颜色和强度。在血清素合成水平和褪黑素合成的日节律过程中发生了明显变化。
Zotero 插件
