Jiang Nan, Cao Jing, Wang Zixu, Dong Yulan, Chen Yaoxing
Laboratory of Anatomy of Domestic Animals, College of Veterinary Medicine, China Agricultural University , Haidian, Beijing, China.
Laboratory of Anatomy of Domestic Animals, College of Veterinary Medicine, Qingdao Agricultural University , Qingdao, China.
Chronobiol Int. 2020 Aug;37(8):1140-1150. doi: 10.1080/07420528.2020.1754846. Epub 2020 Apr 20.
The avian pineal gland is an independent molecular oscillator that receives external light information that regulates the synthesis and secretion of melatonin. Arylalkylamine N-acetyltransferase plays an important role in the pineal gland by controlling the rhythmic production of melatonin. Previous study showed that monochromatic light influences the secretion of melatonin, which is regulated by the molecular circadian clock genes in chick pineal gland. This study was designed to investigate the effect of monochromatic light on the circadian rhythm of levels of c, clock protein (CLOCK and BMAL1), c, and opsins (c; c; c) in chick pineal gland. A total of 240 post-hatching day (P) 0 broiler chickens were reared under white (WL), red (RL), green (GL), and blue light (BL) with light (L)-dark (D) cycle of 12L:12D for 14 d. The results show significant circadian rhythms in the expression of c, CLOCK, BMAL1, c, c, c, and c, but not for c under RL. Compared with WL, GL increased the level of mRNA, while RL decreased it. Meanwhile, CLOCK and BMAL1 proteins were expressed at high levels in GL. Furthermore, the peak of the 24 h pattern of mRNA in GL was earlier than that of in WL, RL, and BL. These results demonstrated that monochromatic light affects the daily expression of in the chick pineal gland via the biological clock. GL activates the transcription of , while RL suppresses the transcription of . Meanwhile, GL appears to induce the peak of mRNA in advance to affect the transmission of light. Thus, monochromatic light regulates in the chick pineal gland by affecting the levels of clock regulators via entraining the expression of pineal gland opsins.
禽类松果体是一个独立的分子振荡器，它接收调节褪黑素合成与分泌的外部光信息。芳基烷基胺N - 乙酰基转移酶通过控制褪黑素的节律性产生在松果体中发挥重要作用。先前的研究表明，单色光会影响褪黑素的分泌，而褪黑素的分泌受雏鸡松果体中分子生物钟基因的调节。本研究旨在探讨单色光对雏鸡松果体中时钟蛋白（CLOCK和BMAL1）、视蛋白（c；c；c）水平昼夜节律的影响。总共240只孵化后第0天（P0）的肉鸡在白色（WL）、红色（RL）、绿色（GL）和蓝光（BL）下饲养，光照（L）-黑暗（D）周期为12L:12D，持续14天。结果显示，c、CLOCK、BMAL1、c、c、c和c的表达存在显著的昼夜节律，但在RL条件下c没有。与WL相比，GL增加了mRNA水平，而RL降低了该水平。同时，CLOCK和BMAL1蛋白在GL中高表达。此外，GL中mRNA的24小时模式峰值早于WL、RL和BL中的峰值。这些结果表明，单色光通过生物钟影响雏鸡松果体中[此处原文可能有缺失信息]的每日表达。GL激活[此处原文可能有缺失信息]的转录，而RL抑制其转录。同时，GL似乎提前诱导mRNA峰值以影响光的传递。因此，单色光通过夹带松果体视蛋白的表达来影响时钟调节因子水平，从而调节雏鸡松果体中的[此处原文可能有缺失信息]。
