卡介苗（BCG）疫苗免疫后溃疡分枝杆菌特异性免疫应答。
Mycobacterium ulcerans-specific immune response after immunisation with bacillus Calmette-Guérin (BCG) vaccine.
机构信息
Infectious Diseases Group, Murdoch Children's Research Institute, Parkville, Victoria, Australia; Infectious Diseases Unit, The Royal Children's Hospital Melbourne, Parkville, Victoria, Australia.
Department of Paediatrics, The University of Melbourne, Parkville, Victoria, Australia; University College London Great Ormond Street Institute of Child Health, University College London, London, United Kingdom; Department of Paediatric Infectious Diseases and Immunology, Evelina London Children's Hospital, Guy's and St. Thomas' National Health Service Foundation Trust, London, United Kingdom.
出版信息
Vaccine. 2021 Jan 22;39(4):652-657. doi: 10.1016/j.vaccine.2020.11.045. Epub 2020 Dec 25.
BACKGROUND
Bacillus Calmette-Guérin (BCG) vaccine provides partial protection against Buruli ulcer caused by Mycobacterium ulcerans in epidemiological studies. This study aimed to quantify M. ulcerans-specific immune responses induced by BCG immunisation.
METHODS
Intracellular cytokine analysis of in-vitro experiments done 10 weeks after BCG immunisation in 130 Australian infants randomised to one of three BCG vaccine strains given either at birth (BCG-Denmark, BCG-Japan, or BCG-Russia) or at two months of age (BCG-Denmark).
RESULTS
Proportions of polyfunctional CD4 T-cells were higher in M. ulcerans-stimulated compared to unstimulated control samples. These proportions were not influenced by the vaccine strain or timing of the immunisation. The M. ulcerans-specific immune responses showed similar patterns to those observed in M. tuberculosis-stimulated samples, although they were of lower magnitude.
CONCLUSIONS
Our data show that BCG immunisation induces M. ulcerans-specific immune responses in infants, likely explaining the cross-protective effect observed in epidemiological studies. (ACTRN12608000227392).
背景
卡介苗（BCG）疫苗在流行病学研究中为预防由溃疡分枝杆菌引起的伯氏疏螺旋体溃疡提供了部分保护。本研究旨在定量分析卡介苗免疫接种诱导的溃疡分枝杆菌特异性免疫应答。
方法
对 130 名澳大利亚婴儿进行了体外实验，这些婴儿在出生时（BCG-丹麦、BCG-日本或 BCG-俄罗斯）或两个月大时（BCG-丹麦）随机接种了三种 BCG 疫苗株中的一种，在接种后 10 周进行了细胞内细胞因子分析。
结果
与未刺激对照样本相比，受溃疡分枝杆菌刺激的多能性 CD4 T 细胞的比例更高。这些比例不受疫苗株或免疫接种时间的影响。与受结核分枝杆菌刺激的样本相比，溃疡分枝杆菌特异性免疫应答呈现出相似的模式，尽管其幅度较低。
结论
我们的数据表明，BCG 免疫接种可在婴儿中诱导溃疡分枝杆菌特异性免疫应答，这可能解释了流行病学研究中观察到的交叉保护作用。（ACTRN12608000227392）。
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