Kutahya University of Health Sciences, Department of Neurosurgery, Kutahya, Turkey.
Turk Neurosurg. 2021;31(2):206-210. doi: 10.5137/1019-5149.JTN.30316-20.1.
To investigate the cytotoxic effects of boron application at different doses on U-87 MG glioblastoma cells.
The T98G (ATCC® CRL-1690?) glioblastoma cell strain used in the study was acquired from the American Type Culture Collection (ATCC) (Manassas, USA). Boric acid solution was prepared by mechanical mixing in the medium. Afterwards, 2.5 mM, 25 mM and 50 mM boron were each added to U87-MG glioblastoma cells and incubated for 48 hours. The cytotoxic effects on the cells was determined using the MTT (Methylthiazole diphenyl tetrazolium) test 48 hours after boron application.
IC50 value was detected as 17 mM in the 48-hour boric acid application on U-87 MG glioblastoma cells.
Boron treatment might be an effective approach for glioblastoma.
研究不同剂量硼应用对 U-87 MG 神经胶质瘤细胞的细胞毒性作用。
本研究中使用的 T98G（ATCC® CRL-1690？）神经胶质瘤细胞株购自美国模式培养物集存库（ATCC）（美国马纳萨斯）。硼酸溶液通过机械混合在培养基中制备。之后，将 2.5mM、25mM 和 50mM 硼分别添加到 U87-MG 神经胶质瘤细胞中，并孵育 48 小时。硼应用 48 小时后，使用 MTT（甲基噻唑二苯基四唑）试验测定对细胞的细胞毒性作用。
在 U-87 MG 神经胶质瘤细胞中，48 小时硼酸应用的 IC50 值为 17mM。
硼处理可能是神经胶质瘤的一种有效方法。
