Zhang Hong-Na, Cui Na, Shen Hong-Miao
College of Biological Science and Engineering, Hebei University of Economics and Business, Shijiazhuang 050061, China.
Huan Jing Ke Xue. 2021 Jan 8;42(1):211-220. doi: 10.13227/j.hjkx.202005305.
Owing to the long residence times of water, water reservoirs readily contribute to the accumulation of antibiotic resistant gene (ARG). It is of great public health significance to explore bacterial communities, antibiotic resistomes, and the potential public health risks of water reservoirs. In this study, metagenomic sequencing was used to analyze and compare the bacterial communities, ARG profiles, ARG-horizontal transfer, and ARG-carrying pathogens in the water and sediments of the Dongping Lake Reservoir in the dry and the wet seasons. Compared with that of the sediments, the results showed that both the bacterial communities and ARG profiles in the water were significantly influenced by the seasons, and the total ARG abundance in the dry season was significantly higher than that in the wet season. The total ARG abundance in the sediments was higher than that in water, but the horizontal transfer potential of ARG in the water was higher than that in the sediment. A total of 377 ARG subtypes belonging to 20 ARG types were found in this study. Bacitracin and vancomycin resistance genes were the main ARG types in the water and sediments, respectively, and Proteobacteria and Actinobacteria were the major ARG-carrying bacteria in the water and sediments, respectively. In addition, 30 clinical pathogens carrying ARGs were identified, including , , , and More importantly, two concurrently carried virulence factor and ARG. In summary, this study revealed that a variety of ARG types existed in the Dongping Lake Reservoir, which has posed potential public health risks by contributing to the horizontal transfer of ARG and the accumulation of clinical pathogens. Therefore, it is necessary to regularly monitor the bacterial community and ARG profile in various water bodies.
由于水体的停留时间长，水库很容易导致抗生素抗性基因（ARG）的积累。探索水库中的细菌群落、抗生素抗性组以及潜在的公共卫生风险具有重大的公共卫生意义。在本研究中，采用宏基因组测序技术分析和比较了东平湖水库存水和沉积物在枯水期和丰水期的细菌群落、ARG谱、ARG水平转移以及携带ARG的病原体。结果表明，与沉积物相比，水中的细菌群落和ARG谱均受季节显著影响，枯水期的总ARG丰度显著高于丰水期。沉积物中的总ARG丰度高于水中，但水中ARG的水平转移潜力高于沉积物。本研究共发现20种ARG类型的377个ARG亚型。杆菌肽和万古霉素抗性基因分别是水中和沉积物中的主要ARG类型，变形菌门和放线菌门分别是水中和沉积物中主要的携带ARG细菌。此外，鉴定出30种携带ARG的临床病原体，包括 、 、 、 和 。更重要的是，两种 同时携带毒力因子和ARG。总之，本研究表明东平湖水库存在多种ARG类型，通过促进ARG的水平转移和临床病原体的积累带来了潜在的公共卫生风险。因此，有必要定期监测各类水体中的细菌群落和ARG谱。
