School of Engineering and Materials Science, Queen Mary University of London, London E1 4NS, U.K.
Institute for Complex Molecular Systems, Laboratory of Macromolecular and Organic Chemistry, Eindhoven University of Technology, P.O. Box 513, Eindhoven 5600 MB, The Netherlands.
Biomacromolecules. 2021 Feb 8;22(2):661-670. doi: 10.1021/acs.biomac.0c01486. Epub 2020 Dec 29.
In this work, we describe the physicochemical characterization of amphiphilic glycopolymers synthesized copper(0)-mediated reversible-deactivation radical polymerization (Cu-RDRP). Depending on the chemical composition of the polymer, these glycopolymers are able to form multi-chain or single-chain polymeric nanoparticles. The folding of these polymers is first of all driven by the amphiphilicity of the glycopolymers and furthermore by the supramolecular formation of helical supramolecular stacks of benzene-1,3,5-tricarboxamides (BTAs) stabilized by threefold hydrogen bonding. The obtained polymeric nanoparticles were subsequently evaluated for their lectin-binding affinity toward a series of mannose- and galactose-binding lectins surface plasmon resonance. We found that addition of 2-ethylhexyl acrylate to the polymer composition results in compact particles, which translates to a reduction in binding affinity, whereas with the addition of BTAs, the relation between the nature of the particle and the binding ability system becomes more unpredictable.
在这项工作中，我们描述了通过铜（0）介导的可逆失活自由基聚合（Cu-RDRP）合成的两亲性糖聚合物的物理化学特性。根据聚合物的化学组成，这些糖聚合物能够形成多链或单链聚合物纳米颗粒。这些聚合物的折叠首先是由糖聚合物的两亲性驱动的，其次是由苯-1,3,5-三羧酸酰胺（BTAs）的螺旋超分子堆叠的超分子形成驱动的，BTAs 由三重氢键稳定。随后，通过表面等离子体共振评估了所得聚合物纳米颗粒对一系列甘露糖和半乳糖结合凝集素的凝集素结合亲和力。我们发现，将 2-乙基己基丙烯酸酯添加到聚合物组成中会导致颗粒紧凑，从而降低结合亲和力，而添加 BTAs 后，颗粒性质与结合能力之间的关系变得更加不可预测。
