用于凝集素识别的糖聚合物接枝聚合物颗粒

Glycopolymer-Grafted Polymer Particles for Lectin Recognition.

作者信息

Kohri Michinari, Taniguchi Tatsuo, Kishikawa Keiki

机构信息

Division of Applied Chemistry and Biotechnology, Graduate School of Engineering, Chiba University, 1-33 Yayoi-cho, Inage-ku, Chiba, 263-8522, Japan.

出版信息

Methods Mol Biol. 2016;1367:137-47. doi: 10.1007/978-1-4939-3130-9_11.

DOI:10.1007/978-1-4939-3130-9_11

PMID:26537470

Abstract

Glycopolymers bearing carbohydrates have an advantage in protein recognition that is attributable to the multivalent effect (cluster effect) of side-chain carbohydrates. A variety of surface-modified polymer particles have been prepared concurrently with the development of new synthetic technology. Here we describe a synthetic method of glycopolymer-grafted polymer particles by surface-initiated living radical polymerization, i.e., atom-transfer radical polymerization (ATRP) and photoiniferter polymerization, for specific lectin recognition.

摘要

带有碳水化合物的糖聚合物在蛋白质识别方面具有优势，这归因于侧链碳水化合物的多价效应（簇效应）。随着新合成技术的发展，人们同时制备了多种表面改性的聚合物颗粒。在此，我们描述了一种通过表面引发的活性自由基聚合，即原子转移自由基聚合（ATRP）和光引发转移终止剂聚合，制备用于特异性凝集素识别的糖聚合物接枝聚合物颗粒的合成方法。