Jiangsu Provincial Key Lab of Solid Organic Waste Utilization, Jiangsu Collaborative Innovation Center of Solid Organic Wastes, Educational Ministry Engineering Center of Resource-saving Fertilizers, Nanjing Agricultural University, Nanjing 210095, Jiangsu, People's Republic of China.
Shandong Institute for Product Quality Inspection, People's Republic of China.
Bioresour Technol. 2021 Mar;323:124599. doi: 10.1016/j.biortech.2020.124599. Epub 2020 Dec 23.
Multiple types of biochemical parameters were determined in the course of the composting process with rice straw and Chinese traditional medicine residues as substrates. The water-soluble fractions (WSFs) were analyzed by excitation-emission-matrix fluorescence (EEM-FL), and the maximum P value (1.2) was observed in thermophilic phase (THP). Bacterial community analysis results indicated that the genera with the capacity of degrading lignocellulose dominated in mesophilic phase (MEP) and THP. The metabolic pathways based on KEGG analysis revealed that the amino acid, carbohydrate and energy metabolism pathways in THP were higher than the other two phases. The correlation analysis between EEM-FL and the bacterial community revealed that the genera with high abundances in the THP were significantly positively correlated with fulvic acid-like materials and humic acid-like organics. The quantification results of the lignocellulose-degrading genes in different phases further verified the key functional bacteria obtained by correlation analysis during the composting process.
在以稻草和中药渣为基质的堆肥过程中，测定了多种类型的生化参数。通过激发-发射矩阵荧光（EEM-FL）分析了水溶性部分（WSFs），在高温期（THP）观察到最大 P 值（1.2）。细菌群落分析结果表明，具有木质纤维素降解能力的属在中温期（MEP）和 THP 中占优势。基于 KEGG 分析的代谢途径表明，THP 中的氨基酸、碳水化合物和能量代谢途径高于其他两个阶段。EEM-FL 与细菌群落的相关分析表明，THP 中丰度较高的属与富里酸类物质和腐殖酸类有机物呈显著正相关。不同阶段木质纤维素降解基因的定量结果进一步验证了堆肥过程中相关分析得到的关键功能细菌。
