Li Yan, Li Jun, Chang Yuan, Li Ruoqi, Zhou Kaiyun, Zhan Yabin, Wei Renyue, Wei Yuquan
Central Laboratory, Haikou Affiliated Hospital of Central South University Xiangya School of Medicine, Haikou, China.
Haikou City Key Laboratory of Clinical Medicine, Haikou, China.
Front Microbiol. 2023 Sep 28;14:1281633. doi: 10.3389/fmicb.2023.1281633. eCollection 2023.
The study aimed to compare the differences in organic fractions transformation, humus components and bacterial community dynamics during manure composting from different sources, and to identify the key biotic and abiotic factors driving the humification process. Five types of manure [pig manure (PM), cow dung (CD), sheep manure (SM), chicken manure (CM), and duck manure (DM)] were used as raw materials for 30 days composting. The results showed the obvious difference of organic fractions decomposition with more cellulose degradation in CD and SM composting and more hemicellulose degradation in PM and CM composting. Composting of PM and CD contained significantly higher humus fractions than the other composts. Fluorescence spectra indicated that SM composting tended to form structurally stable humic acid fractions, while CM and DM tended to form structurally complex fulvic acid fractions. Pearson correlation analysis showed that humification process of composts in category A (PM, CD) with higher humification degree than category B (SM, CM, and DM) was positively correlated with lignin and hemicellulose degradation. Bioinformatics analysis found that promoted the degradation of hemicellulose and the conversion of fulvic to humic acid in the composts of category A, and in category B, , , and were key genera for humic acid formation. Network analysis indicated that bacterial interaction patterns had obvious differences in composting with different humus and humification levels.
该研究旨在比较不同来源粪便堆肥过程中有机组分转化、腐殖质成分和细菌群落动态的差异,并确定驱动腐殖化过程的关键生物和非生物因素。使用五种类型的粪便[猪粪(PM)、牛粪(CD)、羊粪(SM)、鸡粪(CM)和鸭粪(DM)]作为原料进行30天的堆肥。结果表明,有机组分分解存在明显差异,CD和SM堆肥中纤维素降解较多,PM和CM堆肥中半纤维素降解较多。PM和CD堆肥中的腐殖质组分明显高于其他堆肥。荧光光谱表明,SM堆肥倾向于形成结构稳定的腐殖酸组分,而CM和DM倾向于形成结构复杂的富里酸组分。Pearson相关性分析表明,腐殖化程度高于B类(SM、CM和DM)的A类(PM、CD)堆肥的腐殖化过程与木质素和半纤维素降解呈正相关。生物信息学分析发现,促进了A类堆肥中半纤维素的降解以及富里酸向腐殖酸的转化,而在B类堆肥中,、和是腐殖酸形成的关键属。网络分析表明,不同腐殖质和腐殖化水平的堆肥中细菌相互作用模式存在明显差异。