Bogiel Tomasz, Prażyńska Małgorzata, Kwiecińska-Piróg Joanna, Mikucka Agnieszka, Gospodarek-Komkowska Eugenia
Microbiology Department, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toruń, 9 Maria Skłodowska-Curie Street, 85-094 Bydgoszcz, Poland.
Antibiotics (Basel). 2020 Dec 24;10(1):8. doi: 10.3390/antibiotics10010008.
is one of the most commonly isolated bacteria from clinical specimens, with increasing isolation frequency in nosocomial infections. Herein, we investigated whether antimicrobial-resistant strains, e.g., metallo-beta-lactamase (MBL)-producing isolates, may possess a reduced number of virulence genes, resulting from appropriate genome management to adapt to a changing hospital environment. Hospital conditions, such as selective pressure, may lead to the replacement of virulence genes by antimicrobial resistance genes that are crucial to survive under current conditions. The study aimed to compare, using PCR, the frequency of the chosen enzymatic virulence factor genes (alkaline protease-, elastase B-, neuraminidases- and , and both variants of phospholipase C- and ) to MBL distribution among 107 non-duplicated carbapenem-resistant isolates. The gene encoding alkaline protease was noted with the highest frequency (100%), while the neuraminidase-1 gene was observed in 37.4% of the examined strains. The difference in and prevalence amongst the MBL-positive and MBL-negative strains, was statistically significant. Although virulence is generally more likely determined by the complex regulation of the virulence gene expression, herein, we found differences in the prevalence of various virulence genes in MBL-producers.
是临床标本中最常分离出的细菌之一，在医院感染中的分离频率不断增加。在此，我们研究了抗菌耐药菌株，例如产金属β-内酰胺酶（MBL）的分离株，是否可能由于适当的基因组管理以适应不断变化的医院环境而导致毒力基因数量减少。医院环境条件，如选择性压力，可能导致毒力基因被对抗菌药物耐药的基因所取代，而这些耐药基因对于在当前条件下生存至关重要。该研究旨在使用聚合酶链反应（PCR）比较107株非重复的耐碳青霉烯分离株中所选酶促毒力因子基因（碱性蛋白酶、弹性蛋白酶B、神经氨酸酶以及磷脂酶C的两种变体）的频率与MBL分布情况。编码碱性蛋白酶的基因出现频率最高（100%），而在所检测菌株中37.4%观察到神经氨酸酶-1基因。MBL阳性和MBL阴性菌株之间在[具体基因名称1]和[具体基因名称2]流行率上的差异具有统计学意义。尽管[细菌名称]毒力通常更可能由毒力基因表达的复杂调控所决定，但在此我们发现产MBL菌株中各种毒力基因的流行率存在差异。
