Department of Studies in Biotechnology, University of Mysore, Manasagangotri, Mysore 570006, India.
Agilent Technologies India Pvt. Ltd., Bangalore 560048, India.
Cells. 2020 Dec 22;10(1):1. doi: 10.3390/cells10010001.
There is an urge for traditional herbal remedies as an alternative to modern medicine in treating several ailments. is one such plant, used traditionally to treat several diseases. In several reports, there are findings related to the use of this plant extract that demonstrate its therapeutic value. However, very few attempts have been made to identify the extensive metabolite composition of this plant. Here, we performed metabolite profiling and identification from the bark of by extracting the sample in organic and aqueous solvents. The organic and aqueous extracts were fraction-collected using the Agilent 1260 Analytical Scale Fraction Collection System. Each of the fractions was analyzed on Liquid Chromatogaphy/Quadrupole Time-of-Flight LC/Q-TOF and Gas Chromatography/Quadrupole Time-of-Flight GC/instruments. The Liquid Chromatography/Mass Spectrometry (LC/MS) analyses were performed using Hydrophilic Ineraction Liquid Chromatography (HILIC), as well as reversed-phase chromatography using three separate, orthogonal reverse phase columns. Samples were analyzed using an Agilent Jet Stream (AJS) source in both positive and negative ionization modes. The compounds found were flavonoids, fatty acids, sugars, and terpenes. Eighty-one secondary metabolites were identified as having therapeutic potential. The data produced was against the METLIN database using accurate mass and/or MS/MS library matching. Compounds from that could not be identified by database or library matching were subsequently searched against the ChemSpider) database of over 30 million structures using MSMS data and Agilent MSC software.In order to identify compounds generated by GC/MS, the data were searched against the AgilentFiehn GCMS Metabolomics Library as well as the Wiley/NIST libraries.
人们渴望用传统草药来替代现代医学治疗多种疾病。就是这样一种植物，传统上被用于治疗多种疾病。在一些报告中，有关于使用这种植物提取物的研究结果表明其具有治疗价值。然而，很少有尝试去鉴定这种植物的广泛代谢成分。在这里，我们通过提取植物的有机和水溶液来进行代谢物的分析和鉴定。利用安捷伦 1260 分析规模的馏分收集系统对有机和水溶液提取物进行馏分收集。用液相色谱/四极杆飞行时间质谱联用仪（LC/Q-TOF）和气相色谱/四极杆飞行时间质谱联用仪（GC/Q-TOF）对各个馏分进行分析。采用亲水相互作用液相色谱（HILIC）以及三种不同的正交反相色谱柱进行反相色谱分析。使用安捷伦喷气喷雾（AJS）源在正负离子模式下进行 LC/MS 分析。鉴定出的化合物有黄酮类、脂肪酸、糖和萜类化合物。共鉴定出 81 种具有治疗潜力的次生代谢产物。使用精确质量和/或 MS/MS 库匹配的方法，将生成的数据与 METLIN 数据库进行对比。通过数据库或库匹配无法鉴定的化合物，随后使用 MSMS 数据和安捷伦 MSC 软件，在超过 3000 万种结构的 ChemSpider 数据库中进行搜索。为了鉴定 GC/MS 生成的化合物，使用安捷伦的 Fiehn GCMS 代谢组学库和 Wiley/NIST 库对数据进行搜索。
