Welu Gebrelibanos Gebremichael, Yimer Ebrahim M, Hailu Haftom Gebregergs, Bhoumik Dayananda, Lema Mehari Meles
Department of Pharmacology and Toxicology, College of Health Sciences, Mekelle University, Mekelle, Ethiopia.
Pharmacy and Toxicology Unit, Department of Pharmacy, College of Health Sciences, Aksum University, Aksum, Ethiopia.
Evid Based Complement Alternat Med. 2020 Dec 10;2020:1718708. doi: 10.1155/2020/1718708. eCollection 2020.
(Lamiaceae) has been traditionally used for the treatment of various ailments, including body swelling and urine retention. The present study aimed to evaluate the diuretic activity of a crude extract and solvent fractions of the root bark of . . The coarsely powdered root bark of was extracted by a cold maceration method using 80% methanol. A portion of the extract was fractionated based on the polarity index of solvents to obtain chloroform, ethyl acetate, and aqueous fractions. To investigate the diuretic activity of the plant, rats were divided into fifteen groups. The normal control groups received either water or 2% tween 80, the standard group received furosemide (10 mg/kg), and the test groups were administered the hydromethanolic extract and solvent fractions at the doses of 100, 200, and 400 mg/kg by the oral route. The urine volume, urine pH, urine, and serum electrolytes were determined and compared with the standard and normal control groups.
The crude hydromethanolic extract, ethyl acetate, and chloroform fractions induced significant diuresis at a dose of 400 mg/kg ( < 0.001) compared to the aqueous fraction. The hydromethanolic extract at 200 mg/kg and 400 mg/kg also caused noticeable diuresis ( < 0.001) compared to the standard, furosemide. Rats treated with hydromethanolic extract, ethyl acetate, and chloroform fractions showed delayed onset and prolonged diuresis in a dose-dependent fashion compared to the aqueous fraction ( < 0.05). The hydromethanolic extract and solvent fractions produced the highest saliuretic and natriuretic index compared to the standard, furosemide. The crude hydromethanolic extract also failed to produce any sign of toxicity up to 2000 mg/kg.
From this study, the hydromethanolic extract and ethyl acetate fraction of the root bark of produced a prominent diuretic effect in rats.
唇形科植物传统上用于治疗各种疾病，包括身体肿胀和尿潴留。本研究旨在评估[植物名称]根皮粗提物及溶剂萃取物的利尿活性。[植物名称]的根皮粗粉采用冷浸法用80%甲醇进行提取。提取物的一部分根据溶剂的极性指数进行分馏，得到氯仿、乙酸乙酯和水相馏分。为研究该植物的利尿活性，将大鼠分为15组。正常对照组给予水或2%吐温80，标准组给予呋塞米（10 mg/kg），试验组经口给予不同剂量（100、200和400 mg/kg）的甲醇提取物及溶剂萃取物。测定尿量、尿液pH值、尿液及血清电解质，并与标准组和正常对照组进行比较。
与水相馏分相比，甲醇粗提物、乙酸乙酯和氯仿馏分在400 mg/kg剂量时引起显著利尿作用（P<0.001）。与标准药物呋塞米相比，200 mg/kg和400 mg/kg的甲醇提取物也引起明显利尿作用（P<0.001）。与水相馏分相比，用甲醇提取物、乙酸乙酯和氯仿馏分处理的大鼠利尿作用起效延迟且持续时间延长，呈剂量依赖性（P<0.05）。与标准药物呋塞米相比，甲醇提取物及溶剂萃取物产生最高的利盐和利钠指数。甲醇粗提物在高达2000 mg/kg时也未产生任何毒性迹象。
本研究表明，[植物名称]根皮的甲醇提取物和乙酸乙酯馏分对大鼠有显著利尿作用。
