Alehagen Urban, Shamoun Levar, Dimberg Jan Ingvar, Wågsäter Dick
Division of Cardiovascular Medicine, Department of Medicine and Health Sciences, Faculty of Health Sciences, Linköping University, SE-581 85 Linköping, Sweden.
Division of Medical Diagnostics, Department of Laboratory Medicine, Jönköping County, SE-553 05 Jönköping, Sweden.
Exp Ther Med. 2021 Feb;21(2):127. doi: 10.3892/etm.2020.9559. Epub 2020 Dec 7.
One of the major causes of mortality in the western hemisphere is cardiovascular disease. Therefore, a variety of markers to identify those at risk are required. Interleukin-32 (IL-32) is a cytokine that is associated with inflammation. The aim of the current study was to investigate variations in single nucleotide polymorphisms (SNPs) of IL-32 and plasma expression, and their associations with mortality. A population of 486 elderly community-living persons were evaluated. The participants were followed for 7.1 years and underwent a clinical examination and blood sampling. SNP analyses of IL-32 rs28372698 using allelic discrimination and plasma measurement of IL-32, using ELISA, were performed. During the follow-up period, 140 (28.8%) all-cause and 87 (17.9%) cardiovascular deaths were registered. No significant difference between mortality and plasma concentration of IL-32 was observed. The A/A genotype group exhibited significantly higher all-cause mortality (P=0.036), and an almost two-fold increased risk in a multivariate Cox regression model for all-cause and cardiovascular mortality. A highly significant difference in all-cause and cardiovascular mortality between the A/A and the T/T groups was demonstrated (P=0.015 resp. P=0.014). In the present study, the cytokine IL-32 was demonstrated to have prognostic information, with an increased risk of all-cause and cardiovascular mortality for those with the A/A genotype rs28372698 of IL-32. The A/A genotype could therefore be regarded as a possible biomarker for mortality risk that may be used to offer optimized cardiovascular patient handling in the future. However, the present study sample was small, and the results should be regarded as hypothesis-generating.
西半球主要的死亡原因之一是心血管疾病。因此，需要各种标志物来识别高危人群。白细胞介素-32（IL-32）是一种与炎症相关的细胞因子。本研究的目的是调查IL-32单核苷酸多态性（SNP）的变异及其血浆表达，以及它们与死亡率的关联。对486名社区居住的老年人进行了评估。对参与者进行了7.1年的随访，并进行了临床检查和血液采样。采用等位基因鉴别法对IL-32 rs28372698进行SNP分析，并采用酶联免疫吸附测定法（ELISA）检测IL-32的血浆水平。在随访期间，记录了140例（28.8%）全因死亡和87例（17.9%）心血管死亡病例。未观察到死亡率与IL-32血浆浓度之间存在显著差异。A/A基因型组的全因死亡率显著更高（P=0.036），在全因和心血管死亡率的多变量Cox回归模型中，风险几乎增加了两倍。A/A组和T/T组在全因和心血管死亡率方面存在高度显著差异（分别为P=0.015和P=0.014）。在本研究中，细胞因子IL-32被证明具有预后信息，对于IL-32 rs28372698为A/A基因型的个体，全因和心血管死亡风险增加。因此，A/A基因型可被视为一种可能的死亡风险生物标志物，未来可用于优化心血管疾病患者的治疗。然而，本研究样本量较小，结果应视为提出假设。
