Hunan Cancer Hospital, The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, China.
Biomed Res Int. 2020 Dec 7;2020:3981529. doi: 10.1155/2020/3981529. eCollection 2020.
The increased risk and poor survival outcome of cervical adenocarcinoma (CAC) demand for effective early diagnostic biomarkers that can predict the disease progression and outcome. The purpose of this study was to investigate the value of methylation status of and in the detection and prognosis of CAC.
We performed a quantitative methylation-specific polymerase chain reaction in 205 cervical paraffin-embedded specimens (175 CACs, 30 noncancer cervical tissues). Overall and progression-free survival (OS and PFS, respectively) rates were calculated and compared using the Kaplan-Meier method. The prognostic value of and on CAC patients was assessed by the Cox regression model. A mathematical formula combining , , and age was constructed for survival prediction.
The methylation status of and was higher in CAC tissues than in noncancer cervical tissues. In addition, -positive CAC patients showed a higher 5-year OS rate than -negative patients. In CAC patients with smaller tumor size (<4 cm), the -positive group showed a higher 5-year PFS rate than the -negative group. In the algorithm combining , , and age, the low-risk group showed a better 5-year OS and PFS rate than the high-risk group.
and methylation levels are higher in CAC than in normal cervical tissues and are potential biomarkers for monitoring CAC prognosis.
宫颈腺癌（CAC）的风险增加和生存预后不良，需要有效的早期诊断生物标志物，以预测疾病的进展和结局。本研究旨在探讨 和 在 CAC 检测和预后中的甲基化状态的价值。
我们对 205 例石蜡包埋的宫颈标本（175 例 CAC，30 例非癌宫颈组织）进行了定量甲基化特异性聚合酶链反应。采用 Kaplan-Meier 法计算并比较总生存（OS）和无进展生存（PFS）率。采用 Cox 回归模型评估 和 对 CAC 患者的预后价值。构建了一个包含 、 、年龄的数学公式，用于预测生存。
CAC 组织中 和 的甲基化状态高于非癌宫颈组织。此外， -阳性 CAC 患者的 5 年 OS 率高于 -阴性患者。在肿瘤较小（<4cm）的 CAC 患者中， -阳性组的 5 年 PFS 率高于 -阴性组。在包含 、 、年龄的算法中，低危组的 5 年 OS 和 PFS 率均优于高危组。
和 甲基化水平在 CAC 中高于正常宫颈组织，是监测 CAC 预后的潜在生物标志物。
