萝卜块根组织特异性 RNA 分析的激光捕获显微切割优化方案
An Optimized Protocol of Laser Capture Microdissection for Tissue-Specific RNA Profiling in a Radish Tap Root.
机构信息
School of Biological Sciences, Seoul National University, Seoul 08826, Korea.
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 34141, Korea.
出版信息
STAR Protoc. 2020 Sep 17;1(3):100110. doi: 10.1016/j.xpro.2020.100110. eCollection 2020 Dec 18.
Laser capture microdissection (LCM) coupled with transcriptome profiling is a powerful technique that allows for tissue-specific gene expression analysis in a complex system. One major challenge in using this technique is to obtain RNA without compromising its integrity. Here, we present a protocol optimized for radish root tissue sections using Steedman's wax embedding to obtain high-quality RNA suitable for next-generation sequencing analysis. For complete details on the use and execution of this protocol, please refer to Hoang et al. (2020).
激光捕获显微切割（LCM）与转录组谱分析相结合是一种强大的技术，可在复杂系统中进行组织特异性基因表达分析。使用该技术的主要挑战之一是在不损害其完整性的情况下获得 RNA。在这里，我们使用 Steedman 的蜡包埋优化了适用于萝卜根组织切片的方案，以获得适合下一代测序分析的高质量 RNA。有关此方案的使用和执行的完整详细信息，请参见 Hoang 等人。（2020）。
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