制备液滴嵌入囊泡以模拟细胞脂滴。
Making Droplet-Embedded Vesicles to Model Cellular Lipid Droplets.
机构信息
Laboratoire de Physique de l'École Normale Supérieure, ENS, Université PSL, CNRS, Sorbonne Université, Université de Paris, 75005 Paris, France.
出版信息
STAR Protoc. 2020 Sep 25;1(3):100116. doi: 10.1016/j.xpro.2020.100116. eCollection 2020 Dec 18.
We present a reproducible protocol to prepare droplet-embedded vesicles (DEVs) consisting of an oil droplet embedded within a phospholipid bilayer. This model system mimics a cellular lipid droplet (LD) in physical contact with the endoplasmic reticulum (ER) bilayer. It has the advantage that the lipid composition and the biophysical properties of the droplet and the bilayer are controlled and tunable. DEVs can be used to study LD biogenesis factors and determinants of protein binding between ER and LD interfaces. For complete details on the use and execution of this protocol, please refer to Chorlay and Thiam (2020) and Santinho et al. (2020).
我们提出了一个可重现的方案来制备液滴包埋囊泡（DEVs），其由一个油滴嵌入磷脂双层内组成。这个模型系统模拟了与内质网（ER）双层物理接触的细胞脂滴（LD）。它的优点是可以控制和调整液滴和双层的脂质组成和生物物理性质。DEVs 可用于研究 LD 生物发生因子和 ER 与 LD 界面之间蛋白质结合的决定因素。有关此方案使用和执行的完整详细信息，请参考 Chorlay 和 Thiam（2020）和 Santinho 等人（2020）的文章。
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