研究 HIV-1 包膜糖蛋白功能的方案。

Division of Infectious Diseases and International Medicine, Department of Medicine, University of Minnesota, Minneapolis, MN 55455, USA.

Microbiology, Immunology, and Cancer Biology Graduate Program, University of Minnesota, Minneapolis, MN 55455, USA.

STAR Protoc. 2020 Oct 14;1(3):100133. doi: 10.1016/j.xpro.2020.100133. eCollection 2020 Dec 18.

HIV-1 envelope glycoproteins (Envs) bind to CD4 receptor and CCR5/CXCR4 coreceptor and mediate viral entry (Feng et al., 1996; Herschhorn et al., 2016, 2017; Kwong et al., 1998). HIV-1 Envs are the sole target of neutralizing antibodies and a main focus of vaccine development (Flemming et al., 2018). Here, we provide a step-by-step protocol to measure Env sensitivity to ligands, cold, and small molecules, as well as to study viral infectivity and to dissect parameters affecting HIV-1 Env function. For complete details on the use and execution of this protocol, please refer to Harris et al. (2020).

HIV-1 包膜糖蛋白（Env）与 CD4 受体和 CCR5/CXCR4 辅助受体结合，并介导病毒进入（Feng 等人，1996；Herschhorn 等人，2016，2017；Kwong 等人，1998）。HIV-1 Env 是中和抗体的唯一靶标，也是疫苗开发的主要焦点（Flemming 等人，2018）。在这里，我们提供了一个逐步的方案来测量 Env 对配体、冷和小分子的敏感性，以及研究病毒感染力和剖析影响 HIV-1 Env 功能的参数。有关此方案使用和执行的完整详细信息，请参见 Harris 等人（2020）。