临床前模型中的连续循环游离 DNA 评估

Serial Cell-free DNA Assessments in Preclinical Models.

机构信息

Princess Margaret Cancer Center, University Health Network, 101 College Street, Toronto, ON M5G 1L7, Canada.

Department of Medical Biophysics, University of Toronto, 101 College Street, Toronto, ON M5G 1L7, Canada.

出版信息

STAR Protoc. 2020 Oct 20;1(3):100145. doi: 10.1016/j.xpro.2020.100145. eCollection 2020 Dec 18.

DOI:10.1016/j.xpro.2020.100145

PMID:33377039

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7757313/

Abstract

Studying circulating cell-free DNA (cfDNA) release within preclinical model systems provides opportunities to investigate the mechanisms and kinetics underlying this process under various conditions. We present a detailed protocol for longitudinal evaluation of cfDNA release through (1) seeding of cancer cell lines and establishment of xenograft tumors, (2) treatment of cancer cells and xenograft tumors, (3) serial collection of cell line media and xenograft blood, and (4) processing and isolation of cfDNA for (5) quantification of cfDNA by quantitative PCR. For complete details on the use and execution of this protocol please refer to Rostami et al. (2020).

摘要

研究循环无细胞 DNA (cfDNA) 在临床前模型系统中的释放，为在各种条件下研究该过程的机制和动力学提供了机会。我们提出了一个详细的方案，通过（1）癌细胞系的播种和异种移植瘤的建立，（2）癌细胞和异种移植瘤的处理，（3）癌细胞系培养基和异种移植瘤血液的连续采集，以及（4）cfDNA 的处理和分离，用于（5）通过定量聚合酶链反应对 cfDNA 进行定量。有关该方案的使用和执行的完整详细信息，请参见 Rostami 等人（2020 年）。