Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN 37232, USA.
Department of Pharmacology, Vanderbilt University, Nashville, TN 37232, USA.
STAR Protoc. 2020 Dec 21;2(1):100220. doi: 10.1016/j.xpro.2020.100220. eCollection 2021 Mar 19.
Patient-derived tumor organoid cultures are an essential and innovative methodology for translational research. However, current techniques to establish these cultures are cumbersome, expensive, and often require irreplaceable clinical tissue from surgery or core biopsies. Fine-needle aspiration (FNA) provides a minimally invasive biopsy technique commonly performed in clinical settings. Here, we provide a protocol for FNA. We have found that FNA provides a cost-effective, rapid, and streamlined method for tissue acquisition for cancer organoid culture. For complete details on the use and execution of this protocol, please refer to Lee et al. (2020) and Vilgelm et al. (2020).
患者来源的肿瘤类器官培养是转化研究的重要和创新方法。然而，目前建立这些培养物的技术繁琐、昂贵，并且通常需要手术或核心活检中不可替代的临床组织。细针穿刺（FNA）提供了一种常见于临床环境中的微创活检技术。在这里，我们提供了一种 FNA 的方案。我们发现 FNA 为癌症类器官培养提供了一种具有成本效益、快速和简化的组织获取方法。有关此方案使用和执行的完整详细信息，请参见 Lee 等人（2020 年）和 Vilgelm 等人（2020 年）。
