促黄体生成素对肾脏的促生长活性：直接刺激培养的大鼠肾皮质细胞的DNA合成。

Renotropic activity of lutropin: direct stimulation of DNA synthesis of cultured rat renal cortical cells.

作者信息

Nomura K, Horiba N, Sato Y, Ujihara M, Demura H, Shizume K

机构信息

Department of Medicine, Tokyo Women's Medical College, Japan.

出版信息

Biochem Biophys Res Commun. 1988 Jan 15;150(1):506-10. doi: 10.1016/0006-291x(88)90549-9.

DOI:10.1016/0006-291x(88)90549-9

PMID:3337728

Abstract

Two differently purified ovine lutropin (LH) preparations were studied to see if they stimulated [3H] thymidine incorporation into cultured rat renal cortical cells. Both preparations showed a dose-dependent (0.11-1 ng/ml), time-dependent (peaking at 18 h), serum-dependent (7.5% of fetal calf serum or 10% castrated-hypo-physectomized rat serum) renotropic effect. Ovine TSH and FSH failed to mimic this specific, renotropic effect. We concluded that LH directly stimulates renal DNA synthesis in cooperation with other serum factor's.

摘要

对两种不同纯化程度的绵羊促黄体生成素（LH）制剂进行了研究，以观察它们是否能刺激[3H]胸腺嘧啶核苷掺入培养的大鼠肾皮质细胞。两种制剂均显示出剂量依赖性（0.11 - 1 ng/ml）、时间依赖性（在18小时达到峰值）、血清依赖性（胎牛血清的7.5%或去势 - 垂体切除大鼠血清的10%）的促肾生长作用。绵羊促甲状腺激素（TSH）和促卵泡激素（FSH）未能模拟这种特异性的促肾生长作用。我们得出结论，LH与其他血清因子协同直接刺激肾脏DNA合成。